Background: Neurokinin B; an endogenous decapeptide, mediates its reproductive physiological actions through gonadotropin releasing hormone. Despite the potential role of Neurokinin B on seminal vesicles, its effects on seminal vesicles in adult male mammals remain elusive. We aimed to investigate the potentials of variable doses of Neurokinin B, its agonist and antagonist on histomorphology and expression of NK3R on seminal vesicles, and secretory activity of seminal vesicles in adult male rats. Methods: Adult male Sprague Dawley rats (n=10 in each group) were administered intraperitoneally with Neurokinin B in three variable doses: 1 μg, 1 ηg and 10 ρg while, Senktide (Neurokinin B agonist) and SB222200 (Neurokinin B antagonist) in 1 μg doses consecutively for 12 days. After 12 days of peptide treatment, half of the animals (n=05) in each group were sacrificed while remaining half (n=05) were kept for another 12 days without any treatment to investigate treatment reversal. Seminal vesicles were dissected and excised tissue was processed for light microscopy, immunohistochemistry and estimation of seminal fructose levels. Results: Treatment with Neurokinin B and Senktide significantly increased while SB222200 slightly decrease the seminal vesicles weight, epithelial height and seminal fructose levels as compared to control. Light microscopy revealed increased epithelial height and epithelial folding as compared to control in all Neurokinin B and Senktide treated groups while decreased in SB222200. Effects of various doses of Neurokinin B, Senktide and SB222200 on seminal vesicles weight, epithelial height, seminal fructose levels and histomorphology were reversed when rats were maintained without treatments. Immuno-expression of Neurokinin B shows no change in treatment and reversal groups Conclusion: Continuous administration of Neurokinin B and Senktide effect positively while SB222200 have detrimental effects on cellular morphology, epithelial height and seminal fructose levels in seminal vesicles. Effects of peptide treatments depicted a reversal towards control group when rats were kept without any treatment.

中文翻译：

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神经激肽 B 给药可诱导成年大鼠精囊的剂量依赖性增殖

背景：神经激肽B；一种内源性十肽，通过促性腺激素释放激素介导其生殖生理作用。尽管神经激肽 B 对精囊具有潜在作用，但其对成年雄性哺乳动物精囊的影响仍然难以捉摸。我们的目的是研究不同剂量的神经激肽 B 及其激动剂和拮抗剂对成年雄性大鼠精囊组织形态学和 NK3R 表达以及精囊分泌活性的影响。方法：成年雄性 Sprague Dawley 大鼠（每组 n=10）腹腔注射三种不同剂量的神经激肽 B：1 μg、1 ηg 和 10 ρg，同时给予 Senktide（神经激肽 B 激动剂）和 SB222200（神经激肽 B 拮抗剂） 1μg剂量，连续12天。经过 12 天的肽治疗后，每组中一半的动物 (n=05) 被处死，而剩下的一半 (n=05) 则在不接受任何治疗的情况下再保留 12 天，以研究治疗逆转。解剖精囊并处理切除的组织用于光学显微镜检查、免疫组织化学和精液果糖水平的估计。结果：与对照相比，神经激肽 B 和 Senktide 治疗显着增加了精囊重量、上皮高度和精浆果糖水平，而 SB222200 则略有降低。光学显微镜检查显示，与所有神经激肽 B 和 Senktide 治疗组的对照组相比，上皮高度和上皮折叠增加，而 SB222200 则减少。当大鼠不进行治疗时，不同剂量的 Neurokinin B、Senktide 和 SB222200 对精囊重量、上皮高度、精液果糖水平和组织形态学的影响发生逆转。神经激肽 B 的免疫表达在治疗组和逆转组中没有显示变化 结论：连续施用神经激肽 B 和 Senktide 对细胞形态、上皮高度和精囊中的精液果糖水平产生积极影响，而 SB222200 对细胞形态、上皮高度和精液果糖水平产生不利影响。当大鼠未经任何治疗时，肽治疗的效果与对照组相反。