The molecular mechanisms underlying SARS-CoV-2 host cell invasion and life cycle have been studied extensively in recent years, with a primary focus on viral entry and internalization with the aim of identifying antiviral therapies. By contrast, our understanding of the molecular mechanisms involved in the later steps of the coronavirus life cycle is relatively limited. In this review, we describe what is known about the host factors and viral proteins involved in the replication, assembly, and egress phases of SARS-CoV-2, which induce significant host membrane rearrangements. We also discuss the limits of the current approaches and the knowledge gaps still to be addressed.

近年来，人们对 SARS-CoV-2 宿主细胞侵袭和生命周期的分子机制进行了广泛研究，主要关注病毒进入和内化，目的是确定抗病毒疗法。相比之下，我们对冠状病毒生命周期后期涉及的分子机制的理解相对有限。在这篇综述中，我们描述了 SARS-CoV-2 复制、组装和流出阶段所涉及的宿主因子和病毒蛋白的已知情况，这些阶段会诱导显着的宿主膜重排。我们还讨论了当前方法的局限性和仍有待解决的知识差距。