Phalaenopsis ‘Sogo Vivien’ is a hybrid orchid that can produce a mutant with foliar variegation. Variegation can be divided into structural-type and pigment-type variegation. Foliar variegation was mostly caused by mutations in plastid genes. Even though there are various studies on foliar variegation in other plants, studies on foliar variegation in orchids are quite limited. This study aimed to determine the variegated leaf phenotype characteristics and their cause in P. ‘Sogo Vivien’. This study observed the anatomy of variegated leaves by comparing the green and white parts of the leaf, measured the concentration of chlorophyll pigments, and detected leaf starch content between both parts. In addition, polymorphism analysis was also performed on the VAR2 gene partial sequences. Based on leaf anatomy structure, the difference between variegated zones is only in the number of chloroplasts, the diameter of parenchyma cells, and chloroplasts. The chloroplast diameter within the white part is smaller than the green part. The chloroplasts’ diameters in the green part are two times larger than the white part, with four times more in amount. The two parts were formed due to differences in the distribution pattern of functional chloroplasts in the leaves. The concentration of chlorophyll in the green part is higher than in the white part, a characteristic of the chlorophyll-type variegation. The green parts have more starch grains than the white parts. Stomata distance between variegated zones on the abaxial side is different, and it was suggested as a distinctive character between variegated zones. A thymine insertion in exon 1 contributed to nonsense and missense mutations on the VAR2 gene isolated from the white part. This mutation caused the amino acid motif to change from KAKFQ to KA—Q. This mutation is suggested to cause the variegated leaf phenomenon in P. ‘Sogo Vivien’.

蝴蝶兰“Sogo Vivien”是一种杂交兰花，可以产生具有叶杂色的突变体。杂色可分为结构型杂色和色素型杂色。叶杂色主要是由质体基因突变引起的。尽管对其他植物的叶杂色有各种研究，但对兰花叶杂色的研究相当有限。本研究旨在确定P的杂色叶表型特征及其原因。“崇光维维安”。这项研究通过比较叶子的绿色和白色部分来观察杂色叶子的解剖结构，测量叶绿素色素的浓度，并检测这两个部分之间的叶子淀粉含量。此外，还对VAR2基因部分序列进行多态性分析。根据叶子的解剖结构，杂色区之间的差异仅在于叶绿体的数量、薄壁细胞的直径和叶绿体。白色部分的叶绿体直径小于绿色部分。绿色部分的叶绿体直径是白色部分的两倍，数量是白色部分的四倍。这两个部分是由于叶子中功能性叶绿体的分布模式不同而形成的。绿色部分的叶绿素浓度高于白色部分，这是叶绿素型杂色的特征。绿色部分比白色部分含有更多的淀粉粒。远轴侧杂色带之间的气孔距离不同，这被认为是杂色带之间的显着特征。外显子 1 中的胸腺嘧啶插入导致从白色部分分离的VAR2基因发生无义和错义突变。该突变导致氨基酸基序从 KAKFQ 变为 KA-Q。该突变被认为是导致P的杂色叶现象的原因。“崇光维维安”。