Gerontology research on anti-aging interventions with drugs could be an answer to age-related diseases, aiming at closing the gap between lifespan and healthspan. Here, we present two methods for assaying chronological lifespan in human cells: (1) a version of the classical outgrowth assay with quantitative assessment of surviving cells and (2) a version of the PICLS method (propidium iodide fluorescent-based measurement of cell death). Both methods are fast, simple to conduct, cost-effective, produce quantitative data for further analysis and can be used with diverse human cell lines. Whereas the first method is ideal for validation and testing the post-intervention reproductive potential of surviving cells, the second method has true high-throughput screening potential. The new technologies were validated with known anti-aging compounds (2,5-anhydro-d-mannitol and rapamycin). Using the high-throughput screening method, we screened a library of 162 chemical entities and identified three compounds that extend the longevity of human cells.

中文翻译：

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人体细胞 PICLS 是第一个用于鉴定可延长寿命的新型化合物的高通量筛选方法

老年学研究药物抗衰老干预措施可能是解决与年龄有关的疾病的方法，旨在缩小寿命和健康寿命之间的差距。在这里，我们提出了两种测定人类细胞按时间顺序排列的寿命的方法：(1) 一种对存活细胞进行定量评估的经典生长测定法，以及 (2) 一种 PICLS 方法（基于碘化丙啶荧光的细胞死亡测量） ）。这两种方法都快速、操作简单、经济高效，可产生用于进一步分析的定量数据，并且可用于多种人类细胞系。第一种方法非常适合验证和测试存活细胞的干预后生殖潜力，而第二种方法则具有真正的高通量筛选潜力。新技术已使用已知的抗衰老化合物（2,5-脱水-d-甘露醇和雷帕霉素）进行了验证。使用高通量筛选方法，我们筛选了 162 种化学实体的库，并鉴定了三种可延长人体细胞寿命的化合物。