Research into phytase production is useful for improving the efficiency of animal production, reducing environmental impact, and contributing to the development of sustainable and efficient animal production systems. This study aims to investigate the potential of yeast strains for phytase biosynthesis in nutrient media. Phytase is a phosphomonoesterase (E.C 3.1.3.8) catalyzing in a ladder-like manner the dephosphorylation of phytic acid and its salts, with various resulting myo-inositol phosphates and phosphoric acid. Yeasts of the genera Saccharomyces, Zygosaccharomyces, Candida, and Pichia were evaluated in a two-step screening procedure for phytase production. One hundred and eighteen strains were screened in the first stage, which was conducted on four types of solid culture media containing calcium phytate as the selected background. On PSM medium, many strains were found to form halos as early as the 24th hour of development. Several strains with significant potential for enzyme production were evaluated in the second step of the screening. It was conducted in a liquid culture medium. In conclusion, the strain C. melibiosica 2491 was selected for further studies when cultured in a YPglu culture medium. Further research will focus on finding suitable conditions that increase the biosynthesis of the enzyme, which is of significant technological and practical interest for animal nutrition.

对植酸酶生产的研究有助于提高动物生产效率，减少对环境的影响，并有助于发展可持续和高效的动物生产系统。本研究旨在研究酵母菌株在营养培养基中进行植酸酶生物合成的潜力。植酸酶是一种磷酸单酯酶(EC 3.1.3.8)，以梯状方式催化植酸及其盐的去磷酸化，产生各种肌醇磷酸盐和磷酸。在两步筛选程序中评估了酵母属、接合酵母属、念珠菌属和毕赤酵母属的酵母的植酸酶生产情况。第一阶段筛选了118株菌株，以四种含植酸钙的固体培养基为筛选背景。在 PSM 培养基上，许多菌株早在发育第 24 小时就发现形成光晕。在筛选的第二步中评估了几种具有显着产酶潜力的菌株。它是在液体培养基中进行的。总之，选择菌株C. melibiosica 2491 在 YPglu 培养基中培养时进行进一步研究。进一步的研究将集中于寻找增加酶生物合成的合适条件，这对于动物营养具有重要的技术和实际意义。