This study examined the impact of cyclicity (with or without cycle corpus luteum; CL) on oocyte quality and embryonic development in buffaloes. We collected oocytes from the ovaries of slaughtered buffaloes (N = 158 cyclic; n = 316 ovaries and N = 177 acyclic; n = 353 ovaries). Blood progesterone concentration and number of oocytes per ovary were higher in cyclic buffaloes. Cyclic buffalo ovaries produce higher oocytes with I + II and fewer III + IV grades. Oocytes from cyclic buffaloes had a higher maturation rate based on cumulus expansion, cleavage rate and embryo development to the 8-cell, morula and blastocyst stages than acyclic buffaloes. In conclusion, oocytes recovered from the ovaries of the cyclic buffaloes showed improved oocyte competence and subsequent in vitro blastocyst development.

中文翻译：

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改善从循环水牛中回收的卵母细胞的体外囊胚发育

本研究探讨了周期性（有或没有周期黄体；CL）对水牛卵母细胞质量和胚胎发育的影响。我们从屠宰水牛的卵巢中收集卵母细胞（N  = 158 个循环；n  = 316 个卵巢；N  = 177 个非循环；n  = 353 个卵巢）。周期性水牛的血液孕酮浓度和每个卵巢的卵母细胞数量较高。周期性水牛卵巢产生较高的 I + II 级卵母细胞和较少的 III + IV 级卵母细胞。基于卵丘扩张、卵裂率和胚胎发育至8细胞、桑椹胚和囊胚阶段，来自周期性水牛的卵母细胞比非周期性水牛具有更高的成熟率。总之，从循环水牛卵巢中回收的卵母细胞显示出卵母细胞能力的改善以及随后的体外囊胚发育。