The identification of human remains is of utmost importance in a variety of scenarios. One of the primary identification methods is DNA. DNA extraction from human remains could be difficult, particularly in situations where the remains have been exposed to environmental conditions and other insults. Several studies tried to improve extraction by applying different approaches. ForensicGEM Universal (MicroGem) is a single-tube approach to DNA extraction and a temperature-driven method that could have some advantages with respect to previous techniques, among them, reducing the risk of contamination, not requiring specialized equipment, or several steps to perform. The aim of this study was to assess, for the first time, the efficiency of DNA extraction and quality of STR profiles applying the MicroGem protocol and modifications of this protocol from tooth samples in comparison with automatic extraction (AE). Our results indicated that AE and MicroGem performed similar, though with variability depending on the MicroGem modifications, increasing the DNA yield and STR profile quality when DNA is concentrated with Microcon. These findings demonstrated the efficiency of this methodology for DNA extraction from human remains while also providing a simple and quick technique suitable to apply in a variety of forensic scenarios.

中文翻译：

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评估在人类遗骸中应用温度驱动方法进行 DNA 分离和分析的效率

在各种情况下，人体遗骸的识别至关重要。主要的鉴定方法之一是DNA。从人类遗骸中提取 DNA 可能很困难，特别是在遗骸暴露于环境条件和其他侮辱的情况下。几项研究试图通过应用不同的方法来改进提取。ForensicGEM Universal (MicroGem) 是一种单管 DNA 提取方法和温度驱动方法，相对于以前的技术具有一些优势，其中包括降低污染风险、不需要专门设备或执行多个步骤。本研究的目的是首次评估应用 MicroGem 方案以及该方案的修改从牙齿样本中提取 DNA 的效率和 STR 谱的质量，并与自动提取 (AE) 进行比较。我们的结果表明，AE 和 MicroGem 表现相似，但存在差异，具体取决于 MicroGem 修饰，当使用 Microcon 浓缩 DNA 时，提高了 DNA 产量和 STR 谱质量。这些发现证明了这种从人类遗骸中提取 DNA 的方法的效率，同时还提供了一种适合应用于各种法医场景的简单快速的技术。