Estrogens are emergent pollutants found in low levels in water bodies, and for this reason they require concentration for quantification. In this study, aqueous two-phase systems (ATPSs) were used as an alternative strategy to improve hormone detection and quantification from real water samples. In addition, a predictive computational method (COSMO-RS) was used to understand better the migration of hormones between the two phases at the molecular level. Water samples previously analyzed as hormone-free were added to the 250-fold concentrated ATPS, where it was possible to detect and quantify concentrations of these micropollutants in the range of 9.3–29.19 ng L⁻¹.

中文翻译：

---

水性两相系统中的激素浓度：实验和计算分析

雌激素是水体中含量较低的新出现的污染物，因此需要对其进行浓缩以进行定量。在本研究中，水性两相系统 (ATPS) 被用作替代策略，以改进真实水样中的激素检测和定量。此外，还使用预测计算方法（COSMO-RS）在分子水平上更好地了解激素在两个阶段之间的迁移。将之前分析为不含激素的水样添加到 250 倍浓缩的 ATPS 中，可以检测和定量这些微污染物浓度在 9.3–29.19 ng L ^-1范围内。