Batrachochytrium dendrobatidis (Bd) infects amphibians and has been linked to the decline of hundreds of anuran amphibians all over the world. In the province of Groningen in the Netherlands, this fungal pathogen was not detected before this study. To determine whether Groningen was Bd-free, we surveyed 12 locations in this province in 2020 and 2021. Samples were then used to quantify the presence of Bd with a qPCR assay. In total, 2 out of 110 (∼0.02%) collected in 2020 and 11 out of 249 samples collected in 2021 tested positive for Bd. Infected amphibians were found in 4 out of the 12 sites, and the prevalence of Bd was estimated at 4% for both years combined. Our study provides the first record of Bd in Groningen, and we hypothesize that Bd is present throughout the Netherlands in regions currently considered “Bd-free.” Furthermore, we warn scientists and policymakers to be apprehensive when calling a site free from Bd when sampling is limited or not recent.

Batrachochytrium dendrobatidis ( Bd ) 感染两栖动物，并与世界各地数百种无尾两栖动物的数量减少有关。在荷兰格罗宁根省，在这项研究之前并未检测到这种真菌病原体。为了确定格罗宁根省是否不含Bd，我们于 2020 年和 2021 年对该省的 12 个地点进行了调查。然后使用样本通过 qPCR 检测来量化Bd的存在。总体而言，2020 年收集的 110 个样本中有 2 个（约 0.02%）和 2021 年收集的 249 个样本中有 11 个检测出Bd呈阳性。12 个地点中的 4 个地点发现了受感染的两栖动物，两年的Bd流行率估计合计为 4%。我们的研究提供了格罗宁根Bd的第一个记录，我们假设在整个荷兰目前被认为“无Bd ”的地区都存在Bd。此外，我们警告科学家和政策制定者，在采样有限或不是最近采样的情况下，称某个地点不含Bd时要小心谨慎。