The oral cavity contains numerous microorganisms, including antimicrobial-resistant bacteria. These microorganisms can be transmitted via respiratory particles from patients to healthcare providers and vice versa during dental care. We evaluated the spread of Staphylococcus aureus during standardized dental procedures using different scaling devices and rinsing solutions.

During systematic therapy for dental biofilm removal (guided biofilm therapy), using an airflow or ultrasound device to a model simulation head. Staphylococcus aureus suspension was injected into the mouth of the model to mimic saliva. Different suction devices (conventional saliva ejector or a prototype) and rising solutions (water or chlorhexidine) were used. To assess contamination with S. aureus, an air-sampling device was placed near the oral cavity and samples of surface areas were collected.

S. aureus was only detected by air sampling when the conventional saliva ejector with airflow was used. No growth was observed during treatments with the ultrasonic piezo instrument or the prototype suction device. Notably, a rinsing solution of chlorhexidine digluconate decreased the bacterial load compared to water. Surface contamination was rarely detected (1 of 120 samples).

Although our findings indicate potential airborne bacterial transmission during routine prophylactic procedures, specific treatment options during biofilm removal appear to reduce air contamination. These options include ultrasonic piezo devices or the prototype suction device. The use of chlorhexidine reduced the CFU counts of S. aureus detected by air sampling. Surface contamination during dental procedures was a rare occurrence.

口腔中含有大量微生物，包括具有抗药性的细菌。在牙科护理期间，这些微生物可以通过呼吸道颗粒从患者传播到医疗保健提供者，反之亦然。我们使用不同的洁牙设备和冲洗液评估了标准化牙科手术过程中金黄色葡萄球菌的传播情况。

在去除牙齿生物膜的系统治疗（引导生物膜治疗）期间，使用气流或超声设备对模型模拟头进行治疗。将金黄色葡萄球菌悬浮液注入模型的口腔中以模拟唾液。使用不同的抽吸装置（传统的唾液喷射器或原型）和上升溶液（水或氯己定）。为了评估金黄色葡萄球菌的污染情况，将空气采样装置放置在口腔附近并收集表面区域的样本。

当使用带有气流的传统唾液喷射器时，仅通过空气采样检测到金黄色葡萄球菌。在使用超声波压电仪器或原型抽吸装置治疗期间没有观察到生长。值得注意的是，与水相比，二葡萄糖酸氯己定冲洗溶液减少了细菌负荷。很少检测到表面污染（120 个样品中的 1 个）。

尽管我们的研究结果表明在常规预防程序中存在潜在的空气传播细菌传播，但生物膜去除过程中的特定治疗方案似乎可以减少空气污染。这些选项包括超声波压电设备或原型抽吸设备。使用氯己定降低了空气采样检测到的金黄色葡萄球菌CFU 计数。牙科手术期间的表面污染很少发生。