A dualmode tetracycline (TC) assay was developed based on a colloidally stable mixture of TC-specific aptamer stabilized AuNPs (Apt-stabilized AuNPs) and graphite carbon nitride nanosheets (g-CN) for sensitive detection of TC. The presence of TC in the solution could be identified based on the colorimetric and fluorescent signals. This dual-mode assay requires no salt during TC detection and has high sensitivity, allowing detection limits (LOD) of TC to 1.0 × 10 M with a wide dynamic detection range (six orders of magnitude, from 5.0 × 10 to 5.0 × 10 M), and exhibits good selectivity for TC over other metal cations, antibiotics, amino acids, and proteins. The mechanism of this dual-mode assay for TC detection was investigated in detail. Moreover, the amount of TC in actual samples (spiked drinking water and milk) was successfully monitored. The findings provide valuable guidance in developing an advanced platform for detecting TC and other antibiotics.

中文翻译：

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用于四环素检测的高灵敏度双模式测定

基于 TC 特异性适体稳定 AuNP（Apt 稳定 AuNP）和石墨氮化碳纳米片 (g-CN) 的胶体稳定混合物，开发了双模式四环素 (TC) 检测，用于灵敏检测 TC。可以根据比色和荧光信号来识别溶液中 TC 的存在。这种双模式检测在 TC 检测过程中无需加盐，灵敏度高，TC 的检测限 (LOD) 可达 1.0 × 10 M，动态检测范围宽（6 个数量级，从 5.0 × 10 M 到 5.0 × 10 M） ），并且相对于其他金属阳离子、抗生素、氨基酸和蛋白质，TC 具有良好的选择性。详细研究了这种 TC 检测双模式测定的机制。此外，还成功监测了实际样品（添加饮用水和牛奶）中的TC含量。这些发现为开发检测TC和其他抗生素的先进平台提供了宝贵的指导。