The stubby-root nematode genus Paratrichodorus comprises species of plant polyphagous ectoparasitic nematodes distributed worldwide and a few of them are plant virus-vectors (tobravirus). This is one of the most difficult nematode genera in terms of species identification, since it is phenotypically conserved with closely related species and is highly sensitive to the specimen fixation and preservation processes. Using an integrative and multidisciplinary taxonomy framework, we determined species boundaries among the Paratrichodorus populations from a P. allius species-complex sampled in cultivated and natural ecosystems across Spain. We support the hypothesis that nematode diversity of the P. allius species-complex remains underestimated and describe here a total of eight new species on the basis of detailed morphological and molecular characterization of 16 Paratrichodorus populations: Paratrichodorus asidonaensis sp. nov., Paratrichodorus benalupensis sp. nov., Paratrichodorus hinogerus sp. nov., Paratrichodorus litoralis sp. nov., Paratrichodorus paraallius sp. nov., Paratrichodorus rocianerus sp. nov., Paratrichodorus tarifaensis sp. nov., and Paratrichodorus uliaensis sp. nov. This study emphasizes the importance of properly applying a broad taxonomic framework in plant-parasitic nematodes. It is also relevant for plant health, since it correctly delimits species morphologically closed to the virus vector nematode, P. allius, and gives proper molecular markers for its unequivocal identification.

中文翻译：

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揭示 Paratrichodorus allius 群物种复合体中的神秘多样性，以解决该属的八个新物种和分子系统发育的新见解（线虫纲：Trichodoridae）

短根线虫属副毛线虫属包括分布于世界各地的植物多食性外寄生线虫种类，其中一些是植物病毒载体（烟草病毒）。这是物种鉴定中最困难的线虫属之一，因为它与密切相关的物种在表型上保守，并且对标本固定和保存过程高度敏感。利用综合性多学科分类框架，我们从西班牙各地耕作和自然生态系统中采样的 P. allius 物种复合体中确定了 Paratrichodorus 种群之间的物种边界。我们支持这样的假设，即 P. allius 物种复合体的线虫多样性仍然被低估，并根据 16 个 Paratrichodorus 种群的详细形态和分子特征描述了总共 8 个新物种：Paratrichodorus asidonaensis sp。十一月，Paratrichodorus benalupensis sp。十一月，Paratrichodorus hinogerus sp。十一月，Paratrichodorus litoralis sp。十一月，Paratrichodorus paraallius sp。十一月，Paratrichodorus rocianerus sp。十一月，Paratrichodorus tarifaensis sp。nov.，和 Paratrichodorus uliaensis sp。十一月 这项研究强调了在植物寄生线虫中正确应用广泛的分类框架的重要性。它还与植物健康相关，因为它正确地界定了形态上与病毒载体线虫 P. allius 接近的物种，并为其明确的识别提供了适当的分子标记。