Abstract

Innate immunity plays an important role in prevention against pathogenic microorganisms. At present, the significance of its structures during vaccination is widely discussed. The objective of the survey is to study the dynamics of immunological parameters in the immune response to various vaccine types. Animals were vaccinated against anthrax (n = 5) and rabies (n = 5) to analyze the levels of serum natural antibodies in sheep and possible correlations between their parameters and the innate immunity parameters in postvaccintation immune response after blood sample collection (for reference values). On the first day of the immune response, a slight increase in total amount of immunoglobulins and an elevated level of neutrophils in blood of sheep of experimental groups were observed, while the lymphocyte count decreased. On the seventh day, the concentration of total amount of immunoglobulins, involving specific antibodies responding to antigens contained in vaccines against rabies and anthrax, increased to two times its reference values. However, the natural antibody concentration slightly increased only on the first day of the immune response to the anthrax vaccine. Regardless of the vaccine administered to the animals, a negative correlation between the counts of lymphocytes and neutrophils always remained stable (r = –0.88 ± 0.02). A negative correlation between the values for neutrophils and natural antibodies (r = –0.52; r = –0.76) tended to remain in sheep of the experimental groups and in the control (r = –0.48), which can indicate the asynchronous activation of phagocytes and natural antibody synthesis. A correlation between lymphocytes and natural antibodies in the immune response to the rabies (r = 0.5) and anthrax (r = 0.7) vaccines was determined, while the lack of it (r = 0.16) was observed for the control group, which can indicate the response of natural antibodies to a foreign antigen. Correlations between both lymphocytes and natural antibodies (r = 0.7) and lymphocytes and total amount of immunoglobulins (r = 0.8) were higher in the dynamics of immune responses to the anthrax vaccine than that to the rabies vaccine (r = 0.5). Apparently, the natural serum anti-KLH antibodies, including various Ig isotypes, may reflect the innate humoral immune competence.

中文翻译：

---

绵羊免疫反应中天然抗体、一般免疫球蛋白和免疫活性血细胞参数之间的关系

摘要

先天免疫在预防病原微生物方面发挥着重要作用。目前，其结构在疫苗接种过程中的意义被广泛讨论。该调查的目的是研究对各种疫苗类型的免疫反应中免疫学参数的动态。动物接种炭疽疫苗（n =5）和狂犬病疫苗（n =5），分析羊血清天然抗体水平及其参数与采集血样后免疫反应中天然免疫参数之间可能的相关性（仅供参考） ）。免疫反应第一天，实验组羊血液中免疫球蛋白总量略有增加，中性粒细胞水平升高，淋巴细胞计数下降。第七天，免疫球蛋白总量（包括对狂犬病和炭疽疫苗中所含抗原做出反应的特异性抗体）的浓度增加至参考值的两倍。然而，仅在对炭疽疫苗产生免疫反应的第一天，天然抗体浓度略有增加。无论给动物注射哪种疫苗，淋巴细胞和中性粒细胞计数之间的负相关始终保持稳定（r = –0.88 ± 0.02）。中性粒细胞和天然抗体值之间的负相关性 ( r = –0.52; r = –0.76) 在实验组绵羊和对照组 ( r = –0.48)中往往保持不变，这可以表明吞噬细胞的异步激活以及天然抗体的合成。确定了狂犬病 ( r = 0.5) 和炭疽 ( r = 0.7) 疫苗免疫反应中淋巴细胞和天然抗体之间的相关性，而 对照组则观察到缺乏这种相关性 ( r = 0.16)，这可以表明天然抗体对外来抗原的反应。在炭疽疫苗的免疫反应动态中，淋巴细胞和天然抗体（r = 0.7）以及淋巴细胞和免疫球蛋白总量（r = 0.8）之间的相关性高于狂犬病疫苗（r = 0.5）。显然，天然血清抗KLH抗体，包括各种Ig同种型，可能反映先天体液免疫能力。