Acne is a highly prevalent skin disease with a great psychological impact on patients as self-perception, self-confidence, and depression. This work aimed to develop an anti-acne preparation from active anti-bacterial medicinal plants to circumvent the severe side effects and drug resistance commonly reported with topical erythromycin anti-acne preparations. Essential oils: Salvia officinalis L. (sage), Rosmarinus officinalis L. (rosemary), Commiphora myrrha Nees Engl. (myrrh), Origanum majorana L. (marjoram), Pelargonium zonale L. L’Hér. ex Aiton (geranium) and Chrysanthemum morifolium Ramat. (chrysanthemum) were extracted by hydrodistillation and analyzed using gas chromatography/mass spectrometry (GC/MS). The anti-acne activities of the oils against Cutibacterium acnes ATCC 6919 were evaluated by microdilution methods to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The most active essential oils were loaded in a film-forming nanogel prepared with chitosan, pluronic F127 and glycerol in the ratio of 3:1:1, prior to investigation in a murine acne in vivo model. Marjoram and chrysanthemum oils showed the highest antimicrobial activity against C. acnes (MIC = 0.156% v/v and 0.125% v/v, respectively). GC/MS of the actives showed that gamma-terpinene (26.46%) and terpinen-4-ol (22.24%) were the predominant constituents in marjoram, whereas chrysanthenone (32.79%) was the main component in chrysanthemum. The formulated essential oil-loaded film-forming nanogels of both oils exhibited significant anti-acne activity in mice via reducing the bacterial loads, activating the antioxidant nuclear factor erythroid 2–related factor 2 (Nrf2) pathway and inhibiting the inflammatory tumor necrosis factor-alpha (TNF-α) pathway. Further studies should be designed to evaluate the clinical evidence for the use of marjoram and chrysanthemum oil products in acne treatment.

中文翻译：

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牛至和菊花的潜在抗痤疮纳米凝胶配方。精油

痤疮是一种非常普遍的皮肤病，对患者的自我认知、自信和抑郁等心理影响很大。这项工作旨在开发一种由活性抗菌药用植物制成的抗痤疮制剂，以避免外用红霉素抗痤疮制剂常见的严重副作用和耐药性。精油：鼠尾草（Salvia officinalis L.）、迷迭香（Rosmarinus officinalis L.）、没药没药（Commiphora myrma Nees Engl.）(没药)、牛至 (牛至) (马郁兰)、天竺葵 (天竺葵) L. L'Hér。ex Aiton（天竺葵）和 Chrysanthemum morifolium Ramat。（菊花）通过水蒸馏提取并使用气相色谱/质谱 (GC/MS) 进行分析。通过微量稀释方法评估油对痤疮皮肤杆菌 ATCC 6919 的抗痤疮活性，以确定最低抑制浓度 (MIC) 和最低杀菌浓度 (MBC)。在对小鼠痤疮体内模型进行研究之前，将最具活性的精油装入由壳聚糖、普朗尼克 F127 和甘油以 3:1:1 的比例制备的成膜纳米凝胶中。马郁兰和菊花油对痤疮丙酸杆菌表现出最高的抗菌活性（MIC 分别为 0.156% v/v 和 0.125% v/v）。活性成分GC/MS分析表明，马郁兰中的主要成分为γ-萜品烯(26.46%)和萜品烯-4-醇(22.24%)，而菊花中的主要成分为菊酮(32.79%)。两种油配制的负载精油的成膜纳米凝胶通过减少细菌负荷、激活抗氧化核因子红细胞2相关因子2（Nrf2）途径和抑制炎症性肿瘤坏死因子，在小鼠中表现出显着的抗痤疮活性。 α (TNF-α) 途径。应设计进一步的研究来评估使用马郁兰和菊花油产品治疗痤疮的临床证据。