The importance of involving genetic diversity in breeding has been emphasized for the sustainable and stable utilization of crop genetic resources and preparation for unpredictable future environments. The black raspberry fruit from the genus Rubus is highly consumed owing its flavor and health benefits, but the genetic basis of its cultivars is very limited with insufficient genetic information available. Therefore, breeding programs must focus on the exploitation of genetic diversity of wild crop relatives. We assessed the genetic diversity, population differentiation, and genetic structure of 11 wild relatives of R. coreanus Miq. using 10 microsatellite markers to collect valuable genetic information and propose enhanced strategies for development of breeding populations. The observed and expected heterozygosity were 0.493 and 0.520, respectively. Genetic differentiation among the populations was 0.109. The Mantel test confirmed that wild R. coreanus populations differentiated following isolation by distance. Moreover, Bayesian clustering analysis showed that R. coreanus populations consisted of three genetic clusters, distribution of those showed a geographical tendency. Based on the genetic diversity, private allele frequency, and genetic structure results, we suggest to preferentially include the Chiak, Hamyang, and Jeju populations for construction of a breeding population of the Korean black raspberry.

人们强调将遗传多样性纳入育种对于作物遗传资源的可持续和稳定利用以及为不可预测的未来环境做好准备的重要性。悬钩子属的黑树莓果实因其风味和健康益处而被广泛食用，但其品种的遗传基础非常有限，可用的遗传信息不足。因此，育种计划必须注重野生作物近缘种遗传多样性的开发。我们评估了R. coreanus Miq 11 种野生近缘种的遗传多样性、种群分化和遗传结构。使用 10 个微卫星标记来收集有价值的遗传信息，并提出育种种群发展的增强策略。观察到的杂合度和预期杂合度分别为 0.493 和 0.520。群体间的遗传分化为0.109。曼特尔测试证实，野生R. coreanus种群在按距离隔离后会发生分化。此外，贝叶斯聚类分析表明，R. coreanus种群由3个遗传簇组成，其分布呈现地理趋势。根据遗传多样性、私有等位基因频率和遗传结构结果，我们建议优先纳入Chiak、Hamyang和Jeju群体来构建韩国黑树莓育种群体。