The Turiaçu pineapple cultivar produces fruits of high organoleptic value but has few biotechnological studies on seedling production. However, conventional in vitro propagation can affect the photosynthetic potential of plants when transferred to the field, thus mitigating measures should be undertaken to solve this limitation, for example by decreasing carbohydrate concentration in the growth medium, adopting bioreactors of temporary immersion with forced ventilation, and using gas permeable membranes in the culture flask. The present work focused on evaluating the growth and development of plantlets from Ananas comosus [L]. Merr. cultivar Turiaçu, an important but neglected pineapple cultivar, under different sucrose concentrations and cultivation systems. For that, the impact of the photomixotrophic and photoautotrophic growth on morphophysiological responses of plants and survival during the ex vitro acclimatization was assessed. The plants were grown in four cultivation systems: permanent immersion system with sealed flasks (PIS-SF); permanent immersion system with natural ventilation (PIS-NV); single-flask temporary immersion bioreactors (TIS-PF); and twin-flasks temporary immersion bioreactors (TIS-RALM), combined with sucrose concentrations (0, 5.0, 15.0, and 30.0 g L⁻¹). The results indicate that Turiaçu plants have photoautotrophic potential in vitro, as the photochemical efficiency of the plants increased in cultivation systems with TIS –RALM gas exchange without the addition of sucrose. Furthermore, it also improved the performance and hardening of plants in ex vitro conditions, which constitutes a crucial step towards the diffusion of this cultivar.

Turiaçu 菠萝品种生产出具有高感官价值的果实，但对幼苗生产的生物技术研究很少。然而，传统的离体繁殖在转入田间时会影响植物的光合潜力，因此应采取缓解措施来解决这一限制，例如降低生长介质中的碳水化合物浓度、采用临时浸没并强制通风的生物反应器、并在培养瓶中使用透气膜。目前的工作重点是评价凤梨试管苗的生长发育[L]。梅尔。品种Turiaçu，一个重要但被忽视的菠萝品种，在不同的蔗糖浓度和栽培系统下。为此，评估了光合营养和光合自养生长对植物形态生理反应和体外适应期间存活的影响。这些植物在四种栽培系统中生长：带密封烧瓶的永久浸没系统（PIS-SF）；带自然通风的永久浸没系统（PIS-NV）；单瓶临时浸入式生物反应器（TIS-PF）；和双烧瓶临时浸入式生物反应器（TIS-RALM），结合蔗糖浓度（0、5.0、15.0 和 30.0 g L ^-1）。结果表明，Turiaçu 植物在体外具有光自养潜力，因为在不添加蔗糖的情况下，在采用 TIS –RALM 气体交换的栽培系统中，植物的光化学效率有所提高。此外，它还改善了植物在体外条件下的性能和硬化，这是该品种传播的关键一步。