Lipid storage in the form of triacylglycerol (TAG) is essential for insect life, as it enables flight, development, and reproduction. The activity of the lipase brummer (bmm) has been shown to be essential to insects' homeostasis. The objective of this study was to evaluate how bmm expression occurs in Aedes aegypti larvae and adults, and to observe TAG levels during fasting in adult females. The bmm sequence was identified in A. aegypti and exhibited a patatin-like phospholipase domain reinforced by the presence of a catalytic dyad with serine and aspartate residues, revealing a high degree of similarity with other organisms. Bmm expression was differentiated in the larvae and adult fat body (FB) following TAG reserve dynamics. Bmm was expressed three times in larval stages L3, L4, and pupae compared with L1 and L2, which could indicate its role in the maturation of these insects. In the postemergence (PE) and post-blood meal (PBM) FB of adult insects, bmm expression varied over several days. PE adults showed a pronounced bmm increase from the third day onward compared with those not subjected to fasting. This was accompanied by a decrease in TAG from the third day onward, suggesting the participation of bmm. Six hours after blood feeding, TAG levels increased in mosquitos reared in the absence of sucrose, suggesting lipid accumulation to guarantee reproduction. Bmm responded positively to fasting, followed by TAG mobilization in adult FB. During the previtellogenic period, bmm levels responded to low TAG levels, unlike the PBM period.

中文翻译：

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埃及伊蚊（双翅目：蚊科）不同发育阶段（包括禁食的成虫）中布鲁默的差异表达和 TAG 水平

以三酰甘油 (TAG) 形式储存的脂质对于昆虫的生命至关重要，因为它能够实现飞行、发育和繁殖。脂肪酶布鲁默 (bmm) 的活性已被证明对于昆虫的体内平衡至关重要。本研究的目的是评估埃及伊蚊幼虫和成虫中 bmm 表达的情况，并观察成年雌性禁食期间的 TAG 水平。bmm 序列在埃及伊蚊中得到鉴定，并表现出类似 patatin 的磷脂酶结构域，该结构域通过丝氨酸和天冬氨酸残基催化二元体的存在得到增强，揭示了与其他生物体的高度相似性。根据 TAG 储备动态，Bmm 表达在幼虫和成虫脂肪体 (FB) 中存在差异。与 L1 和 L2 相比，Bmm 在幼虫期 L3、L4 和蛹中表达了 3 次，这可能表明其在这些昆虫成熟中的作用。在成年昆虫的出苗后 (PE) 和血粉后 (PBM) FB 中，bmm 表达在几天内发生变化。与未禁食的成年人相比，PE 成年人从第三天起表现出明显的 bmm 增加。从第三天开始，TAG 下降，表明 bmm 的参与。喂血后六小时，在没有蔗糖的情况下饲养的蚊子中，TAG 水平升高，表明脂质积累以保证繁殖。Bmm 对禁食反应积极，随后成人 FB 中的 TAG 动员。与 PBM 时期不同，在卵黄发生前期，bmm 水平对低 TAG 水平有反应。