While dual-display DNA-encoded chemical libraries (DELs) are increasingly employed for ligand discovery, some of their fundamental properties have not yet been studied in-depth. Aided with fluorescence polarization experiments, we demonstrate that dual-display DELs are intrinsically asymmetrical entities, and we deduce practical guidelines to perform better-informed on-DNA hit validation from these libraries.

中文翻译：

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双显示 DNA 编码化学文库的不对称性

虽然双显示 DNA 编码化学文库 (DEL) 越来越多地用于配体发现，但其一些基本特性尚未得到深入研究。借助荧光偏振实验，我们证明了双显示 DEL 本质上是不对称实体，并且我们推断出实用指南，以便从这些库中执行更明智的 DNA 命中验证。