Background

For fully automated detection and quantification of Plasmodium parasites, Sysmex developed the XN-31 hemocytometer. This study investigated whether the XN-31 can also detect and quantify bloodstream form trypanosomes (trypomastigotes).

Methods

Axenic cultures of Trypanosoma brucei brucei were used to prepare two dilution series of trypomastigotes in the whole blood of a healthy donor, which were subsequently examined by the XN-31 as well as by microscopic examination of thin and thick blood films. Trypomastigote intactness during the procedures was evaluated by microscopy.

Results

The XN-31 hemocytometer detected trypomastigotes with a detection limit of 26 trypomastigotes/μL. Scattergram patterns of Trypanosoma and Plasmodium parasites were clearly distinct, but current interpretation settings do not allow the identification of trypomastigotes yet, and therefore, need future refinement.

Conclusion

Proof of concept was provided for an automated fluorescent flow cytometry method that can detect and quantify Plasmodium spp., as well as Trypanosoma brucei trypomastigotes.

中文翻译：

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全自动 Sysmex XN-31 血液分析仪可检测布氏锥虫的血流

背景

为了全自动检测和定量疟原虫寄生虫，Sysmex 开发了 XN-31 血细胞计数器。本研究调查了 XN-31 是否也可以检测和量化锥虫（锥鞭毛体）的血流。

方法

布氏锥虫的无菌培养物用于在健康供体的全血中制备两个稀释系列的锥鞭毛体，随后通过 XN-31 以及薄血膜和厚血膜的显微镜检查进行检查。通过显微镜评估手术过程中锥鞭毛体的完整性。

结果

XN-31血细胞计数器检测锥鞭毛体，检出限为26个锥鞭毛体/μL。锥虫和疟原虫寄生虫的散点图模式明显不同，但目前的解释设置尚不允许识别锥鞭毛体，因此需要未来的改进。

结论

为自动荧光流式细胞术方法提供了概念验证，该方法可以检测和量化疟原虫属以及布氏锥虫锥鞭毛体。