Micro RNAs are known as the main regulator of messenger RNA translation in platelets and have a vital role in process of apoptosis during platelet storage. Our pervious study revealed that the expression of miR-145 and miR-326 changed significantly in platelets under maintenance conditions. This study aimed to evaluate the effect of L-carnitine (LC) as an additive to augment platelet quality by changing the microRNA expression. We used ten platelet concentrate (PC) bags and divided each into two equal parts, LC- treated, and LC free PC. The expression of miR-145 and miR-326 were determined using real-time PCR. Moreover, we measured platelet count, platelet aggregation, platelet viability, and lactate dehydrogenase activity in all samples. The miR-326 expression significantly increased during platelet storage with mean fold changes of 3.2 for the control and 2.5 for LC- treated PC. The mean fold changes in miR-145 expression was less in the control PC (0.52) compared to the LC- treated PC (0.79). Increased levels of platelet count, platelet aggregation, and platelet viability were found in the LC-treated compared to the untreated PC. LC has a protective effect on platelet apoptosis, reduces the expression of apoptotic microRNA, and prevents the reduction of anti-apoptotic microRNA.

中文翻译：

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左旋肉碱通过改变凋亡和抗凋亡相关的 microRNA 有助于增强血小板浓缩物的活力和质量

Micro RNA 被认为是血小板中信使 RNA 翻译的主要调节因子，在血小板储存过程中的细胞凋亡过程中发挥着至关重要的作用。我们之前的研究表明，在维持条件下，血小板中 miR-145 和 miR-326 的表达发生显着变化。本研究旨在评估左旋肉碱 (LC) 作为添加剂通过改变 microRNA 表达来提高血小板质量的效果。我们使用十个浓缩血小板 (PC) 袋，并将每个袋分成两等份：经 LC 处理的 PC 和不含 LC 的 PC。使用实时PCR测定miR-145和miR-326的表达。此外，我们还测量了所有样本中的血小板计数、血小板聚集、血小板活力和乳酸脱氢酶活性。 miR-326 表达在血小板储存期间显着增加，对照的平均倍数变化为 3.2，LC 处理的 PC 的平均倍数变化为 2.5。与 LC 处理的 PC (0.79) 相比，对照 PC (0.52) 中 miR-145 表达的平均倍数变化较小。与未处理的 PC 相比，LC 处理的血小板计数、血小板聚集和血小板活力水平有所增加。 LC对血小板凋亡具有保护作用，降低凋亡microRNA的表达，并阻止抗凋亡microRNA的减少。