Collagen is an essential component of biological tissues with a variety of subtypes. To be able to capture these subtypes, fully exploit the polarization-sensitive light-collagen interactions, and provide comprehensive information of collagen, we integrated polarization-sensitive second-harmonic generation (PSHG) microscopy, polarization-sensitive optical coherence microscopy (PSOCM), and two-photon fluorescence lifetime imaging microscopy into a single-source multimodal system in a simultaneous and spatially co-registered configuration. PSOCM information is used in the PSHG numerical model to enable accurate PSHG analysis of unsectioned fresh tissue. This polarization-sensitive multimodal system provides quantitative multiparametric characterization of collagen and facilitates the fundamental understanding of collagen in the unperturbed tissue microenvironment, which can enable future studies into the role of collagen in various diseases.

中文翻译：

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用于胶原蛋白多参数定量表征的无标记多模态偏振敏感光学显微镜

胶原蛋白是生物组织的重要组成部分，具有多种亚型。为了能够捕获这些亚型，充分利用偏振敏感的光-胶原相互作用，并提供胶原蛋白的全面信息，我们集成了偏振敏感二次谐波发生（PSHG）显微镜、偏振敏感光学相干显微镜（PSOCM）、和双光子荧光寿命成像显微镜以同步和空间共同配准的配置集成到单源多模态系统中。PSOCM 信息用于 PSHG 数值模型，以便能够对未切片的新鲜组织进行准确的 PSHG 分析。这种偏振敏感的多模态系统提供了胶原蛋白的定量多参数表征，并有助于对未受干扰的组织微环境中的胶原蛋白的基本了解，这可以使未来研究胶原蛋白在各种疾病中的作用成为可能。