CRISPR/Cas systems stand out because of simplicity, efficiency, and other superiorities, thus becoming attractive and brilliant gene-editing tools in biomedical field including cancer therapy. CRISPR/Cas systems bring promises for cancer therapy through manipulating and engineering on tumor cells or immune cells. However, there have been concerns about how to overcome the numerous physiological barriers and deliver CRISPR components to target cells efficiently and accurately. In this review, we introduced the mechanisms of CRISPR/Cas systems, summarized the current delivery strategies of CRISPR/Cas systems by physical methods, viral vectors, and nonviral vectors, and presented the current application of CRISPR/Cas systems in cancer clinical treatment. Furthermore, we discussed prospects related to delivery approaches of CRISPR/Cas systems.

中文翻译：

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用于癌症治疗的 CRISPR/Cas 基因编辑和递送系统

CRISPR/Cas系统因其简单、高效等优势而脱颖而出，成为包括癌症治疗在内的生物医学领域有吸引力且出色的基因编辑工具。CRISPR/Cas 系统通过对肿瘤细胞或免疫细胞进行操作和改造，为癌症治疗带来了希望。然而，如何克服众多生理障碍，将CRISPR成分高效、准确地递送至靶细胞一直是人们关注的问题。本文介绍了CRISPR/Cas系统的作用机制，总结了目前CRISPR/Cas系统通过物理方法、病毒载体和非病毒载体的递送策略，并介绍了CRISPR/Cas系统目前在癌症临床治疗中的应用。此外，我们还讨论了与 CRISPR/Cas 系统递送方法相关的前景。