Despite their prevalent use in drug discovery and protein biochemistry, non-canonical amino acids are still challenging to synthesize through purely chemical means. In recent years, biocatalysis has emerged as a transformative paradigm for small molecule synthesis. One strategy to further empower biocatalysis is to use it in combination with modern chemical reactions and take advantage of the strengths of each method to enable access to challenging structural motifs that are previously unattainable using each method alone. In this Mini-Review, we highlight several recent case studies that feature the synergistic use of chemical and enzymatic transformations in one pot to synthesize novel non-canonical amino acids.

中文翻译：

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非经典氨基酸的一锅化学酶法合成

尽管非规范氨基酸在药物发现和蛋白质生物化学中广泛使用，但通过纯化学方法合成仍然具有挑战性。近年来，生物催化已成为小分子合成的变革范式。进一步增强生物催化能力的一种策略是将其与现代化学反应结合使用，并利用每种方法的优势来获得具有挑战性的结构基序，而这些基序以前单独使用每种方法是无法实现的。在这篇小评论中，我们重点介绍了最近的几个案例研究，这些案例研究的特点是协同使用化学和酶促转化在一锅中合成新型非规范氨基酸。