Aedes vectors have rapidly spread to regions without any history of arboviral outbreaks in recent time through various modes and due to their ability to rear in manmade containers. Because Aedes immature species can consistently grow in small-domesticated breeding spots, it is important to perform surveillance during the intermittent periods of outbreaks for vector control and management. Detailed entomo-molecular surveillance was carried during the intermittent periods of dengue affected areas of Odisha State of Eastern India from 2021-2022. Indoor and outdoor breeding sites of the Aedes vector were investigated and water containing immature stages were collected, followed by Aedes species assessment using morphological tools along with allele specific PCR using 18S-rRNA region. Aedes albopictus was found to be the main vector through morpho-molecular investigations. Discarded tyres (58%) were the preferred breeding spots, exhibiting significantly high fecundity (p < 0.05, ANOVA test) among all breeding sites. Therefore, monitoring and targeting the intermittent breeding spots will assist in vector control management.

近年来，伊蚊媒介通过各种方式并由于能够在人造容器中繁殖，已迅速传播到没有任何虫媒病毒爆发历史的地区。由于伊蚊未成熟物种可以在小型驯化繁殖点持续生长，因此在爆发间歇期进行监测对于病媒控制和管理非常重要。2021年至2022年，在印度东部奥里萨邦登革热疫区间歇期进行了详细的昆虫分子监测。对伊蚊媒介的室内和室外繁殖场所进行了调查，并收集了含有未成熟阶段的水，然后使用形态学工具以及使用 18S-rRNA 区域的等位基因特异性 PCR 进行伊蚊物种评估。通过形态分子研究发现白纹伊蚊是主要媒介。废弃轮胎（58%）是首选的繁殖点，在所有繁殖点中表现出显着高的繁殖力（p < 0.05，方差分析测试）。因此，监测和瞄准间歇性繁殖点将有助于病媒控制管理。