Designing advanced stationary phases to improve separation efficiency is essential in capillary electrochromatography. Due to their outstanding performance, covalent organic frameworks have recently demonstrated considerable promise in the field of separation science. Herein, an open-tubular capillary electrochromatography method was reported using porous imine-based covalent organic framework with sufficiently available interaction sites as stationary phase. The imine-based covalent organic framework coated capillary was easily prepared via an in situ growth method at room temperature, and its separation performance was evaluated, indicating the high separation efficiency for three types of analytes, including herbicides, polybrominated dibenzofurans, and bisphenols. Moreover, the imine-based covalent organic framework coated capillary showed good reproducibility and stability, with intraday (n = 3), interday (n = 3), and column-to-column (n = 3) relative standard deviations of retention time and peak areas of less than 5%. The separation efficiency of the coated capillary remained unchanged even after 200 runs and the maximum theoretical plates reached up to 85 595 N/m for 4,4′-ethylidenebisphenol. It was predicted that the imine-based covalent organic framework stationary phase would be a strong contender for chromatographic separation with high efficiency.

中文翻译：

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原位生长亚胺基共价有机骨架作为开管毛细管电色谱分离的固定相

设计先进的固定相以提高分离效率对于毛细管电色谱至关重要。由于其出色的性能，共价有机框架最近在分离科学领域表现出了巨大的前景。在此，报道了一种开管毛细管电色谱方法，使用具有足够可用的相互作用位点的多孔亚胺基共价有机框架作为固定相。亚胺基共价有机骨架涂层毛细管可以在室温下通过原位生长方法轻松制备，并对其分离性能进行评估，表明对除草剂、多溴二苯并呋喃和双酚等三类分析物具有较高的分离效率。此外，亚胺基共价有机框架涂层毛细管表现出良好的重现性和稳定性， 保留时间和柱间相对标准偏差为日内 ( n  = 3)、日间 ( n  = 3) 和柱间 ( n = 3)。峰面积小于5%。即使运行 200 次，涂层毛细管的分离效率仍保持不变，4,4'-亚乙基双酚的最大理论塔板数达到 85 595 N/m。据预测，亚胺基共价有机骨架固定相将成为高效色谱分离的有力竞争者。