Objectives: In this study, we employed an in vitro culturing technique to investigate the impact of p53 on the modulation of growth-associated protein-43 (GAP-43) within the primary cortical neurons of rat specimens. Methods: (1) Within the first 24 hours after birth, the bilateral cortex was extracted from newborn Wistar rats and primary cortical neurons were cultured and identified. (2) The changes in the mRNA and protein expressions of GAP-43 induced by p53 in rat primary cortical neurons cultured in vitro were identified utilizing real-time polymerase chain reaction and western blot techniques Results: (1) Lentiviral transfection of p53 within primary cortical neurons of rats elicited elevated levels of both mRNA and protein expressions of GAP-43, consequently culminating in a noteworthy augmentation of p53 expression. (2) The introduction of a p53 inhibitor in rat primary cortical neurons resulted in a reduction in both mRNA and protein expressions of GAP-43. Conclusion: Within primary rat cortical neurons, p53 has the potential to prompt an augmentation in both the transcriptional and protein expression levels of the GAP-43 protein.

中文翻译：

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P53 诱导大鼠初级皮质神经元中 GAP-43 上调

目的：在本研究中，我们采用体外培养技术来研究 p53 对大鼠标本原代皮质神经元内生长相关蛋白 43 (GAP-43) 调节的影响。方法：（1）生后24小时内，抽取新生Wistar大鼠双侧皮质，培养并鉴定原代皮质神经元。 (2)利用实时聚合酶链反应和western blot技术鉴定p53诱导体外培养的大鼠原代皮层神经元GAP-43 mRNA和蛋白表达的变化结果：(1)原代大鼠原代皮层神经元慢病毒转染大鼠皮质神经元引起 GAP-43 mRNA 和蛋白表达水平升高，最终导致 p53 表达显着增加。 (2)在大鼠原代皮质神经元中引入p53抑制剂导致GAP-43的mRNA和蛋白表达均减少。结论：在原代大鼠皮质神经元中，p53 有可能促进 GAP-43 蛋白的转录和蛋白表达水平增加。