Since 2019, many studies on COVID-19, which has caused extensive damage as a pandemic, have been ongoing worldwide. These include serological and biochemical studies using sera from patients and animal models. Testing with these sera must be performed after the inactivation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Heat treatment, UV irradiation, and/or gamma-ray irradiation are used to inactivate viruses in serum. Determining the inactivation conditions that ensure the inactivation of viruses and minimize the effect on test results after inactivation is important to ensure worker safety and accuracy of test results. In this study, serum samples containing SARS-CoV-2 were subjected to heat, UV irradiation, and gamma irradiation to determine their inactivation conditions. The viral titers were below the detection limit after heating at 56°C for 1 h or 60°C for 15 min, UV-B irradiation with a transilluminator for 30 min, or gamma ray irradiation with ⁶⁰Co at 10 kGy. These results provide useful information for safe serological and biochemical experiments.

自 2019 年以来，针对作为大流行病造成广泛损害的 COVID-19 的许多研究一直在全球范围内进行。其中包括使用患者和动物模型的血清进行血清学和生化研究。必须在严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 灭活后使用这些血清进行检测。热处理、紫外线照射和/或伽马射线照射用于灭活血清中的病毒。确定灭活条件，确保病毒灭活并尽量减少灭活后对检测结果的影响，对于确保工作人员的安全和检测结果的准确性非常重要。在这项研究中，含有 SARS-CoV-2 的血清样本经过加热、紫外线照射和伽马射线照射，以确定其灭活条件。56℃加热1小时或60℃加热15分钟，用透照仪UV-B照射30分钟，或用10kGy的^60Co伽马射线照射后，病毒滴度低于检测限。这些结果为安全血清学和生化实验提供了有用的信息。