Genetic manipulation in mammals has progressed rapidly in the past decade with the advent of CRISPR-Cas gene editing tools, promising profound impacts on the understanding of human development, health and disease. However, many years of research in divergent fields of experimental embryology, genetics, reproduction, molecular biology and transgenic technology laid the groundwork and have played critical roles for this progress. This article details various threads of research and the central role of the laboratory mouse that came together in reaching this point, all from the perspective of a scientist whose research was deeply immersed in the field.

中文翻译：

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小鼠基因操作之路：国际分化学会 Jean Brachet 纪念讲座（2023 年 6 月 21 日在冷泉港实验室发表）

随着 CRISPR-Cas 基因编辑工具的出现，哺乳动物的基因操作在过去十年中取得了迅速进展，有望对人类发育、健康和疾病的理解产生深远影响。然而，多年来在实验胚胎学、遗传学、生殖、分子生物学和转基因技术等不同领域的研究为这一进展奠定了基础并发挥了关键作用。本文详细介绍了各种研究线索以及实验室小鼠在达到这一点上的核心作用，所有这些都是从一位深入研究该领域的科学家的角度出发的。