F1 hybrids derived from a cross between two inbred parental lines often display widespread changes in DNA methylation and gene expression patterns relative to their parents. An emerging challenge is to understand how parental epigenomic differences contribute to these events. Here, we generated a large mapping panel of F1 epigenetic hybrids, whose parents are isogenic but variable in their DNA methylation patterns. Using a combination of multi-omic profiling and epigenetic mapping strategies we show that differentially methylated regions in parental pericentromeres act as major reorganizers of hybrid methylomes and transcriptomes, even in the absence of genetic variation. These parental differentially methylated regions are associated with hybrid methylation remodeling events at thousands of target regions throughout the genome, both locally (in cis) and distally (in trans). Many of these distally-induced methylation changes lead to nonadditive expression of nearby genes and associate with phenotypic heterosis. Our study highlights the pleiotropic potential of parental pericentromeres in the functional remodeling of hybrid genomes and phenotypes.

中文翻译：

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绘制亲本 DMR 预测表观遗传 F1 杂种中局部和远端甲基化组重塑。

源自两个近交亲本系之间杂交的 F1 杂种通常表现出相对于其亲本的 DNA 甲基化和基因表达模式的广泛变化。一个新出现的挑战是了解亲代表观基因组差异如何导致这些事件。在这里，我们生成了 F1 表观遗传杂种的大型图谱，其亲本是同基因的，但 DNA 甲基化模式可变。通过结合多组学分析和表观遗传作图策略，我们发现，即使在没有遗传变异的情况下，亲本着丝粒周围的差异甲基化区域也可以充当杂合甲基化组和转录组的主要重组者。这些亲本差异甲基化区域与整个基因组中数千个目标区域的混合甲基化重塑事件相关，包括局部（顺式）和远端（反式）。许多远端诱导的甲基化变化导致附近基因的非加性表达并与表型杂种优势相关。我们的研究强调了亲本着丝粒周围在杂种基因组和表型功能重塑中的多效性潜力。