In this study, we aimed to demonstrate that a significant increase in dietary iron intake disrupts the regulation of copper availability, ultimately leading to systemic copper deficiency. To investigate this, we conducted experiments using five-week-old male weanling Sprague–Dawley rats fed diets based on AIN-93G with some modifications. These diets featured varying iron content, offering choices of adequate iron (~ 120 μg/g, near the upper limit [UL]) or high iron (~ 7544 μg/g), along with low (~ 0.3 μg/g), adequate (~ 6 μg/g), or high (~ 153 μg/g) levels of dietary copper over a 5-week period. Rats consuming the high-iron diets displayed anemia, reduced copper levels in their organs and feces, and shortened colon lengths. Increased dietary iron intake resulted in an overall reduction in copper distribution within the body, likely leading to severe copper deficiency-related disorders in the experimental rats. However, the physiological disturbances caused by a high-iron diet were prevented when additional copper was included in the rodent diet. Furthermore, high iron intake led to copper deprivation, and high iron consumption resulted in elevated serum cholesterol levels. However, increasing dietary copper consumption led to a decrease in overall serum cholesterol levels. Additionally, serum alkaline phosphate and aspartate aminotransferase levels were increased by high-iron feeding, regardless of dietary copper concentration, while alanine aminotransferase levels decreased.

中文翻译：

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高铁消耗会减少铜的积累和结肠长度，并改变血脂

在这项研究中，我们的目的是证明膳食铁摄入量的显着增加会扰乱铜可用性的调节，最终导致系统性铜缺乏。为了研究这一点，我们使用五周大的雄性断奶 Sprague-Dawley 大鼠进行了实验，这些大鼠的饮食基于 AIN-93G 并进行了一些修改。这些饮食具有不同的铁含量，提供充足铁（~ 120 μg/g，接近上限 [UL]）或高铁（~ 7544 μg/g）以及低铁（~ 0.3 μg/g）、充足铁的选择。 （约 6 微克/克），或 5 周内膳食铜含量较高（约 153 微克/克）。食用高铁饮食的老鼠表现出贫血，器官和粪便中的铜含量降低，结肠长度缩短。膳食铁摄入量的增加导致体内铜分布的总体减少，可能导致实验大鼠出现严重的铜缺乏相关疾病。然而，当啮齿动物饮食中添加额外的铜时，高铁饮食引起的生理紊乱就可以得到预防。此外，高铁摄入量导致铜缺乏，而高铁摄入量导致血清胆固醇水平升高。然而，增加膳食铜摄入量会导致总体血清胆固醇水平下降。此外，无论膳食铜浓度如何，高铁喂养都会增加血清碱性磷酸盐和天冬氨酸转氨酶水平，而丙氨酸转氨酶水平则下降。