Cellular and molecular changes occur during aging, decreasing organ function. The aging process was measured by several biomarkers, including DNA methylation (DNAm), an epigenetic change regulating gene expression, which is highly accurate at predicting biological age. DNAm is heritable and therefore varies between different populations. To assess blood DNA methylation changes as epigenetic clocks in the male and female Egyptian population. Pyrosequencing was used to measure the methylation of nine CpG sites in blood samples from 100 healthy Egyptians (18–69 years) using a cross-sectional study. Two age predicted models based on the ELOVL2 gene were compared in three age categories and correlated in all age groups despite decreasing accuracy with increasing age. The mean absolute deviation (MAD) using the 1st and 2nd age predicted models for 18–40 years was 1.06 and 2.7, respectively; for 41–60 years, it was 4.4 and 3.8, respectively; and for > 60 years, it was 7.7 and 7.0, respectively. No significant differences in DNA methylation were found between the sexes. DNA methylation of the ELOVL2 gene can be used as an accurate biomarker for age estimation. Additionally, this method has the potential to be more accurate than traditional methods of age estimation.

中文翻译：

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ELOVL2 基因的 DNA 甲基化作为埃及人口年龄的表观遗传标记

衰老过程中细胞和分子发生变化，器官功能下降。衰老过程是通过多种生物标志物来测量的，其中包括 DNA 甲基化 (DNAm)，这是一种调节基因表达的表观遗传变化，可以高度准确地预测生物年龄。DNAm 具有遗传性，因此不同人群之间存在差异。评估埃及男性和女性人群中血液 DNA 甲基化变化作为表观遗传时钟。通过横断面研究，焦磷酸测序技术测量了 100 名健康埃及人（18-69 岁）血液样本中 9 个 CpG 位点的甲基化情况。两个基于 ELOVL2 基因的年龄预测模型在三个年龄类别中进行了比较，并在所有年龄组中相关，尽管准确性随着年龄的增加而降低。使用第一和第二年龄预测模型对 18-40 岁的平均绝对偏差 (MAD) 分别为 1.06 和 2.7；41-60岁分别为4.4和3.8；60 年来，该值分别为 7.7 和 7.0。性别之间的 DNA 甲基化没有发现显着差异。ELOVL2 基因的 DNA 甲基化可用作年龄估计的准确生物标志物。此外，该方法有可能比传统的年龄估计方法更准确。