The incidence of Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) was surveyed in production fields of cotton, Gossypium hirsutum L., in Tamil Nadu, India, from December 2021 to March 2022. Whitefly counts from the survey indicated a range of 3.26 to 6.72 adults per 3 leaves. The nymphs and adults collected from the fields were examined, and their taxonomic character was confirmed. The mitochondrial Cytochrome Oxidase subunit I gene (mtCOI) and universal primer-based PCR amplification and sequencing have been employed in molecular techniques for further confirmation. The MEGA-X-created dendrogram evaluated using the maximum likelihood method created a single clade with sub-lineages of the specimens collected during this survey. Overall, the mean (SD) duration of the entire life cycle of A. rugioperculatus was 46.3 ± 2.85 days. In many world regions, these whiteflies have been identified as one of agricultural and horticultural crops’ most destructive insect pests. Alternative hosts close to the production fields may be monitored and managed to prevent pest populations from harboring elsewhere during the non-growing season.

2021 年 12 月至 2022 年 3 月，对印度泰米尔纳德邦的棉花（ Gossypium hirsutum L.）产田的白粉虱（半翅目：粉虱科）发病率进行了调查。调查显示粉虱计数范围为 3.26 至 6.72 只成虫每 3 片叶子。对从田间采集的若虫和成虫进行了检查，并确认了它们的分类学特征。线粒体细胞色素氧化酶亚基 I 基因 (mtCOI) 和基于通用引物的 PCR 扩增和测序已在分子技术中得到进一步证实。使用最大似然法评估的 MEGA-X 创建的树状图创建了一个具有本次调查期间收集的样本子谱系的单一进化枝。总体而言， A. rugioperculatus整个生命周期的平均 (SD) 持续时间为 46.3 ± 2.85 天。在世界许多地区，这些粉虱已被确定为农业和园艺作物最具破坏性的害虫之一。可以对靠近生产田的替代寄主进行监测和管理，以防止害虫种群在非生长季节在其他地方藏匿。