Key message

A complete system of regeneration, via somatic embryogenesis, from the in vitro culture of leaflets explants of young A. aculeata donor-plants has been reported.

Abstract

In the present study, a complete regeneration protocol of Acrocomia aculeata (Jacq.) Lodd. ex Mart., via somatic embryogenesis is reported, and the influence of the genotype and its age on the induction of the embryogenic process determined. Leaflets explants of 4 genotypes, aged 2 and 5 years, were inoculated in the induction medium consisting of salts and vitamins Y3 supplemented with different concentrations of picloram (9.0, 18.0 and 36.0 µM). In the control, no plant growth regulators were added. Picloram concentrations of 18.0 and 36.0 µM induced greater formation of embryogenic calluses in all genotypes studied. However, 2-year-old genotypes had higher percentages of embryogenic calluses. In addition, at the highest concentration of picloram (36.0 µM), 5-year-old genotypes had the highest oxidation rates. Differentiation of somatic embryos was observed in medium supplemented with 9.0 and 18.0 µM picloram and 1 mM putrescine. However, at a concentration of 9.0 µM, the somatic embryos showed a high degree of fusion. Embryogenic lines were only obtained in medium supplemented with 18.0 µM picloram and 1 mM putrescine. Histochemical analysis showed the presence of pectins in embryogenic cultures and starch grains in peripheral regions of embryogenic calluses, which were not directly involved in regeneration. Somatic embryos were converted into plantlets after 90 days in germination medium containing 0.54 µM NAA, 1 mM putrescine and 3.0 g L⁻¹ activated charcoal, highlighting the potential of the propagation system proposed here for clonal propagation of A. aculeata.

中文翻译：

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外植体年龄和基因型驱动 Acrocomia aculeata (Jacq.) Lodd 叶外植体的体细胞胚胎发生。前市场。（槟榔科），一种用于石油生产的替代棕榈作物

关键信息

据报道，通过体细胞胚胎发生，从年轻的A. aculeata供体植物的小叶外植体的体外培养物中获得完整的再生系统。

抽象的

在本研究中，Acrocomia aculeata (Jacq.) Lodd 的完整再生方案。报道了从市场上通过体细胞胚胎发生，并确定了基因型及其年龄对胚胎发生过程诱导的影响。将 2 岁和 5 岁的 4 种基因型的小叶外植体接种到由盐和维生素 Y3 组成的诱导培养基中，并添加不同浓度的毒莠定（9.0、18.0 和 36.0 µM）。在对照中，不添加植物生长调节剂。18.0 和 36.0 µM 浓度的毒莠定在所有研究的基因型中诱导更多的胚胎发生愈伤组织形成。然而，2 岁基因型的胚胎发生愈伤组织的比例较高。此外，在毒莠定最高浓度（36.0 µM）下，5年基因型的氧化率最高。在补充有 9.0 和 18.0 µM 毒莠定和 1 mM 腐胺的培养基中观察到体细胞胚胎的分化。然而，在浓度为 9.0 µM 时，体细胞胚胎表现出高度融合。胚胎发生系仅在补充有 18.0 µM 毒莠定和 1 mM 腐胺的培养基中获得。组织化学分析表明，胚胎发生培养物中存在果胶，胚胎发生愈伤组织周围区域存在淀粉粒，这些区域不直接参与再生。^{体细胞胚在含有0.54 µM NAA、1 mM腐胺和3.0 g L -1}活性炭的发芽培养基中90天后转化为幼苗，突出了此处提出的繁殖系统用于A. aculeata克隆繁殖的潜力。