The duodenum acts as a vital organ that performs fundamental physiological functions like digestion and nutrient absorption. Situated in the lower abdomen, the duodenum is located between the stomach and the jejunum. Usually, the duodenum is divided into four anatomical portions. We here compare paraffin embedded and cryosections of the healthy rabbit duodenum for research purposes. This analysis evaluates the differential outcomes resulting from the application of these fixation methodologies in conjunction with immunohistochemical assays targeting extracellular matrix markers collagen I, collagen III, fibronectin, α-smooth muscle actin (α-SMA), and proliferation marker ki67 as well as inflammatory marker PAR-2. Subsequent recommendations are provided based on our findings. Furthermore, the advantage of an antigen retrieval step in immunohistochemical labelling of paraffin sections was demonstrated and confirmed with an isotype negative control. Basic classical histological stainings as HE, GT and elastin were also performed. Comparison of different stainings and labellings was performed in serial sections, showing that adjacent to the circular muscle of the duodenum, the connective tissue was composed of collagen I and fibronectin, while the artery and vein walls were predominantly α-SMA positive. Moreover, PAR-2 immunohistochemical staining was performed, where particularly a type of gland adjacent to Brunner’s glands showed prominent PAR-2 positive areas, while the Brunner’s glands themselves were PAR-2 negative. Proliferating ki67 positive cells facing the lumen were highly abundant in all kinds of glands except for the Brunner’s glands. This effort serves to furnish the research community with reference imagery pertinent to scientists opting for the rabbit duodenum model. The diversity of staining techniques employed herein establishes a foundational repository of images, primed for comparative analysis against pathological conditions. Furthermore, these images hold the potential to illustrate inter-species variations. For instance, they can be juxtaposed against murine or rat intestinal tracts, or even offer insights into the human context.

十二指肠是执行消化和营养吸收等基本生理功能的重要器官。十二指肠位于下腹部，位于胃和空肠之间。通常，十二指肠分为四个解剖部分。我们在这里比较健康兔子十二指肠的石蜡包埋和冷冻切片以用于研究目的。该分析评估了应用这些固定方法与针对细胞外基质标记物胶原蛋白 I、胶原蛋白 III、纤连蛋白、α-平滑肌肌动蛋白 (α-SMA) 和增殖标记物 ki67 以及炎症的免疫组织化学测定所产生的差异结果。标记 PAR-2。根据我们的发现提供了后续建议。此外，石蜡切片免疫组织化学标记中抗原修复步骤的优势已通过同种型阴性对照得到证明和证实。还进行了基本的经典组织学染色，如 HE、GT 和弹性蛋白。在连续切片中进行不同染色和标记的比较，显示邻近十二指肠环肌的结缔组织由 I 型胶原和纤连蛋白组成，而动脉和静脉壁主要为 α-SMA 阳性。此外，进行了PAR-2免疫组织化学染色，其中特别是与Brunner腺体相邻的一种腺体显示出明显的PAR-2阳性区域，而Brunner腺体本身是PAR-2阴性。除布伦纳腺外，所有腺体中面向管腔的增殖的 ki67 阳性细胞都非常丰富。这项工作旨在为研究界提供与选择兔子十二指肠模型的科学家相关的参考图像。本文采用的染色技术的多样性建立了图像的基础存储库，为针对病理条件的比较分析做好了准备。此外，这些图像有可能说明物种间的差异。例如，它们可以与小鼠或大鼠的肠道并列，甚至可以提供对人类背景的见解。