White adipose tissue (WAT) requires extracellular Ca²⁺ influx for lipolysis, differentiation, and expansion. This partly occurs via plasma membrane Ca²⁺ voltage-dependent channels (CaVs). However, WFA exists in different depots whose function varies with age, sex, and location. To explore whether their CaV expression profiles also differ we used RNAseq and qPCR on gonadal, mesenteric, retroperitoneal, and inguinal subcutaneous fat depots from rats of different ages and sex. CaV expression was found dependent on age, sex, and WFA location. In the gonadal depots of both sexes a significantly lower expression of CaV1.2 and CaV1.3 was seen for adults compared to pre-pubescent juveniles. A lower level of expression was also seen for CaV3.1 in adult male but not female gonadal WFA, the latter of whose expression remained unchanged with age. Relatively little expression of CaV3.2 and 3.2 was observed. In post-pubescent inguinal subcutaneous fat, where the third and fourth mammary glands are located, CaV3.1 was decreased in males but increased in females – thus suggesting that this channel is associated with mammogenesis; however, no difference in intracellular Ca²⁺ levels or adipocyte size were noted. For all adult depots, CaV3.1 expression was larger in females than males – a difference not seen in pre-pubescent rats. These observations are consistent with the changes of CaV3.1 expression seen in 3T3-L1 cell differentiation and the ability of selective CaV3.1 antagonists to inhibit adipogensis. Our results show that changes in CaV expression patterns occur in fat depots related to sexual dimorphism: reproductive tracts and mammogenesis.

白色脂肪组织 (WAT) 需要细胞外 Ca ²⁺流入来进行脂肪分解、分化和扩张。这部分是通过质膜 Ca ²⁺电压依赖性通道 (CaV) 发生的。然而，WFA存在于不同的仓库中，其功能因年龄、性别和地点而异。为了探讨它们的 CaV 表达谱是否也不同，我们对不同年龄和性别的大鼠的性腺、肠系膜、腹膜后和腹股沟皮下脂肪库使用 RNAseq 和 qPCR。CaV 表达依赖于年龄、性别和 WFA 位置。在两性的性腺库中，与青春期前的青少年相比，成年人的 CaV1.2 和 CaV1.3 表达显着降低。成年男性性腺 WFA 中 CaV3.1 的表达水平也较低，但女性性腺 WFA 中没有，后者的表达随着年龄的增长保持不变。观察到相对较少的 CaV3.2 和 3.2 表达。在青春期后腹股沟皮下脂肪中，即第三和第四乳腺所在的位置，CaV3.1在男性中减少，但在女性中增加——因此表明该通道与乳房生成有关；然而，细胞内Ca ²⁺水平或脂肪细胞大小没有差异。对于所有成年大鼠，CaV3.1 的表达在雌性大鼠中高于雄性——这一差异在青春期前的大鼠中未见。这些观察结果与 3T3-L1 细胞分化中观察到的 CaV3.1 表达变化以及选择性 CaV3.1 拮抗剂抑制脂肪形成的能力一致。我们的结果表明，CaV 表达模式的变化发生在与性别二态性相关的脂肪库中：生殖道和乳房发生。