The application of a recombination sensor for real-time detection of Lactate Dehydrogenase (LDH) activities has been demonstrated. LDH activity in biological fluids, particularly in blood serum, is a critical diagnostic indicator of human health. Therefore, the development of methods for monitoring it remains a crucial task in modern bioengineering. The parameter directly measured in real-time is the photocurrent through a deep-barrier structure under light illumination from the region of strong optical absorption in silicon. The detection of enzyme presence can be achieved because the flow of the dehydrogenase reaction (in the presence of LDH activation) is accompanied by changes in charge at the system's interface. Such a change in the effective charge of the reactants can be detected through the amplitude of the sensor structure's photocurrent. The main factor in this approach is the influence on the recombination center parameters at the interface. It has been shown that the combined use of modulated signal illumination and constant illumination allows obtaining additional information about the analyte and reaction kinetics. A promising approach is proposed, which can be considered a simple and sensitive method for real-time detection of lactate dehydrogenase activity.

中文翻译：

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光伏复合传感器作为乳酸脱氢酶活性实时测定系统

重组传感器实时检测乳酸脱氢酶（LDH）活性的应用已经得到证实。生物体液（尤其是血清）中的 LDH 活性是人类健康的重要诊断指标。因此，开发监测方法仍然是现代生物工程的一项重要任务。实时直接测量的参数是在硅中强光吸收区域的光照射下通过深势垒结构的光电流。可以实现酶存在的检测，因为脱氢酶反应的流动（在LDH活化的情况下）伴随着系统界面处的电荷变化。反应物有效电荷的这种变化可以通过传感器结构光电流的幅度来检测。该方法的主要因素是对界面处复合中心参数的影响。已经表明，调制信号照明和恒定照明的组合使用可以获得关于分析物和反应动力学的附加信息。提出了一种有前景的方法，可以被认为是实时检测乳酸脱氢酶活性的简单而灵敏的方法。