Background: Rutin is a natural flavonol that showed excellent antiglycation activity with an IC50 value of 294.5 ± 1.5 μM. In the current study, three selected plant species of Euphorbia, i.e., Euphorbia helioscopia, Euphorbia larica, and Euphorbia wallichii, were analyzed for the quantification of rutin. Methods: The quantification was done through a newly developed method of Emission spectroscopy coupled with Partial Least Square Regression (PLSR) and UV-visible spectroscopy as a parallel cross-validation method. Results: The spectroscopic results indicated the highest rutin concentration in the roots of E. helioscopia (11.25 mg/100 g) followed by roots of E. wallichii (9.93 mg/100 g), leaves of E. helioscopia and the whole plant of E. larica (9.41 mg/100 g). The leaves of E. wallichii (8.66 mg/100 g) were found to contain the lowest concentration of rutin among all the tested samples. Conclusion: The present method is one of the simple, robust, and non-destructive methods to carry out the quantitative estimation of rutin in plants.

中文翻译：

---

通过花光谱和偏最小二乘回归分析对选定大戟属物种中的芦丁（一种抗糖化药物）进行定量

背景：芦丁是一种天然黄酮醇，具有优异的抗糖化活性，IC50 值为 294.5 ± 1.5 μM。在当前的研究中，对三种选定的大戟属植物物种，即大戟、大戟和大戟进行了芦丁的定量分析。方法：通过新开发的发射光谱法与偏最小二乘回归（PLSR）和紫外-可见光谱法作为并行交叉验证方法来完成定量。结果：光谱结果表明，芦丁含量以天麻根部最高（11.25 mg/100 g），其次是华天麻根（9.93 mg/100 g）、天麻叶和天麻全株。 . 落叶松 (9.41 毫克/100 克)。 E. wallichii 的叶子 (8.66 mg/100 g) 被发现含有所有测试样品中最低浓度的芦丁。结论：该方法是一种简单、可靠、无损的植物中芦丁定量测定方法。