Agricultural waste valorisation provides a sustainable solution to waste management and combining waste utilisation with commodity production allows for responsible production processes. Recombinant Aspergillus niger D15 strains expressing fungal endoglucanases (Trichoderma reesei eg1 and eg2 and Aspergillus carneus aceg) were evaluated for the ability to utilise lactose as a carbon source to determine whether dairy waste could be used as a feedstock for enzyme production. The recombinant A. niger D15[eg1]PyrG, D15[eg2]PyrG and D15[aceg]PyrG strains produced maximum endoglucanase activities of 34, 54, and 34 U/mL, respectively on lactose and 23, 27, and 22 U/mL, respectively on whey. The A. niger D15[eg2]PyrG strain was used to optimise the whey medium. Maximum endoglucanase activity of 46 U/mL was produced on 10% whey medium containing 0.6% NaNO3. The results obtained indicate that dairy whey can be utilised as a feedstock for recombinant enzyme production. However, variations in enzyme activities were observed and require further investigation.

中文翻译：

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以乳清作为碳水化合物源培养重组黑曲霉菌株

农业废物增值为废物管理提供了可持续的解决方案，并将废物利用与商品生产相结合，实现负责任的生产过程。对表达真菌内切葡聚糖酶（里氏木霉eg1和eg2以及肉曲霉aceg）的重组黑曲霉D15菌株利用乳糖作为碳源的能力进行了评估，以确定乳品废物是否可以用作酶生产的原料。重组黑曲霉 D15[eg1]PyrG、D15[eg2]PyrG 和 D15[aceg]PyrG 菌株产生的最大内切葡聚糖酶活性分别为 34、54 和 34 U/mL，对乳糖和 23、27 和 22 U/mL mL，分别为乳清。使用黑曲霉 D15[eg2]PyrG 菌株来优化乳清培养基。在含有 0.6% NaNO3 的 10% 乳清培养基上产生的最大内切葡聚糖酶活性为 46 U/mL。获得的结果表明乳清可以用作重组酶生产的原料。然而，观察到酶活性的变化，需要进一步研究。