The emergence of FluidFM technology has presented a novel opportunity for selective aspiration and distribution of liquids at the sub-micron scale. However, precise control over droplet generation volume remains a challenging issue. This study investigates the impact of dynamic parameters between the tip and surface on droplet formation. Initially, we establish a finite element model to simulate the process from the formation of a liquid bridge between the hollow cantilever probe and the substrate to the rupture resulting in droplet formation. Additionally, we propose an image-based method for quantifying droplet volume. Subsequently, we construct a comprehensive experimental framework to delve into the influence of these parameters on microdroplet formation by monitoring the dynamic interaction parameters between the probe tip and the liquid surface. Finally, we optimize the parameters during the droplet generation process, enabling the formation of uniform arrays of microdroplets under controlled experimental conditions. Our study provides a reference for automation parameters of serial experiments and improves experimental throughput.

中文翻译：

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通过流体力显微镜观察动态尖端-表面相互作用对微滴形成的影响

FluidFM 技术的出现为亚微米级液体的选择性吸入和分配提供了新的机会。然而，对液滴生成量的精确控制仍然是一个具有挑战性的问题。本研究研究了尖端和表面之间的动态参数对液滴形成的影响。首先，我们建立了一个有限元模型来模拟从空心悬臂梁探针和基底之间形成液桥到破裂导致液滴形成的过程。此外，我们提出了一种基于图像的方法来量化液滴体积。随后，我们构建了一个综合的实验框架，通过监测探针尖端与液体表面之间的动态相互作用参数来深入研究这些参数对微滴形成的影响。最后，我们优化了液滴生成过程中的参数，从而能够在受控的实验条件下形成均匀的微滴阵列。我们的研究为系列实验的自动化参数提供了参考，提高了实验通量。