The Lung Cancer Compact Panel (compact panel) is a gene panel that can detect driver alterations with high sensitivity in liquid samples, including tumor cells. This study examined the ability of a compact panel to detect genetic mutations in liquid specimens used in clinical practice. Three cohorts, bronchoscopic biopsy forceps washing (washing cohort), pleural effusion (pleural cohort), and spinal fluid (spinal cohort), were analyzed using the compact panel. Liquid samples were added into the GM (GeneMetrics) tubes and analyzed. The washing cohort assessed the concordance rate of gene panel analysis outcomes in tissue specimens derived from the primary tumor. Meanwhile, the pleural cohort investigated the impact of storing specimens for 8 weeks and more on nucleic acid and mutation detection rates. In the washing cohort (n = 79), the concordance rate with mutations detected in tissues was 75/79 (94.9 %). This rate reached 100 % when focusing solely on driver alterations for treatment. The pleural cohort (n = 8) showed no deterioration in nucleic acid quality or quantity after 8 weeks of storage in GM tubes. Similarly, in the spinal cohort (n = 9), spinal fluid with malignant cells exhibited driver alterations similar to those in the primary tumor. These findings underscore the efficacy of the compact panel in accurately identifying genetic mutations in different liquid specimens. The compact panel is a reliable tool for detecting driver alterations in various cytological specimens. Its consistent performance across diverse sample types emphasizes its potential for guiding targeted therapies for patients with lung cancer and enhancing precision medicine approaches.

中文翻译：

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Lung Cancer Compact Panel™ 各类细胞学标本在肺癌患者中的临床应用

肺癌紧凑型面板（compact panel）是一种基因面板，可以高灵敏度地检测液体样本（包括肿瘤细胞）中的驱动基因改变。这项研究检验了紧凑型面板检测临床实践中使用的液体样本中基因突变的能力。使用紧凑面板对三个队列进行分析：支气管镜活检钳清洗（清洗队列）、胸腔积液（胸膜队列）和脊髓液（脊髓队列）。将液体样品添加到 GM (GeneMetrics) 管中并进行分析。清洗队列评估了原发性肿瘤组织标本中基因组分析结果的一致性。同时，胸膜队列研究了保存标本 8 周或更长时间对核酸和突变检出率的影响。在清洗组 (n = 79) 中，与组织中检测到的突变的一致性为 75/79 (94.9%)。当仅关注驾驶员改变进行治疗时，该比率达到 100%。在 GM 管中储存 8 周后，胸膜队列 (n = 8) 显示核酸质量或数量没有恶化。同样，在脊柱队列中（n = 9），含有恶性细胞的脊髓液表现出与原发肿瘤相似的驱动改变。这些发现强调了紧凑面板在准确识别不同液体样本中基因突变方面的功效。紧凑型面板是检测各种细胞学样本中驱动器变化的可靠工具。其在不同样本类型中的一致表现强调了其指导肺癌患者靶向治疗和增强精准医学方法的潜力。