The measurement of neuropeptides using small electrodes for high spatial resolution would provide us with localized information on the release of neuromolecules. The release of Neuropeptide Y (NPY) is related to different neurological diseases such as stress, obesity, and PTSD, among others. In this conference paper, we electrodeposited polypyrrole on carbon fiber microelectrodes in the presence of NPY to develop a molecularly imprinted polypyrrole sensitive to NPY. Optimization of the electrodeposition process resulted in the full coverage of the polymer with nucleation sites on the carbon fiber ridges, achieving completion by the seventh cycle. Electrodeposition was performed for five cycles, and using cyclic voltammetry (CV), we studied the change in the oxidation current peak for polypyrrole due to the presence of NPY. We also observed a change in capacitance due to the presence of NPY, which was studied by electrochemical impedance spectroscopy (EIS). A linear correlation was found between the oxidation peak and the concentration of NPY between 50 ng/mL and 1000 ng/mL. In addition, a linear correlation was also found between microelectrode capacitance and the concentration of NPY between 50 ng/mL and 1000 ng/mL at 100 kHz.

中文翻译：

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碳纤维微电极上分子印迹聚吡咯测量神经肽Y

使用小电极进行高空间分辨率的神经肽测量将为我们提供有关神经分子释放的局部信息。神经肽 Y (NPY) 的释放与压力、肥胖和创伤后应激障碍 (PTSD) 等不同的神经系统疾病有关。在这篇会议论文中，我们在 NPY 存在的情况下将聚吡咯电沉积在碳纤维微电极上，开发出对 NPY 敏感的分子印迹聚吡咯。电沉积工艺的优化导致聚合物完全覆盖碳纤维脊上的成核位点，并在第七个循环时完成。电沉积进行了五个循环，并使用循环伏安法（CV），我们研究了由于 NPY 的存在而导致的聚吡咯氧化电流峰的变化。我们还观察到由于 NPY 的存在而导致的电容变化，这是通过电化学阻抗谱 (EIS) 进行研究的。氧化峰与 NPY 浓度在 50 ng/mL 至 1000 ng/mL 之间呈线性相关。此外，在 100 kHz 下，微电极电容与 NPY 浓度在 50 ng/mL 至 1000 ng/mL 之间也存在线性相关性。