Bean common mosaic virus (BCMV) is a significant pathogen that affects common bean, leading to substantial yield losses and reduced crop quality. To mitigate BCMV attacks, certain genes, including diacylglycerol kinases genes (), genes associated with defense and stress responses (, , and ), as well as genes related to plant defense (1, 2, and 3) play an essential functional role in various stress responses in common bean. In this study, the expression levels of 1235a5b6, , , , 1, 2, and 3 genes were investigated in the leaves of different common bean genotypes under BCMV infection conditions. Through quantitative real-time PCR analysis, we observed varying expression patterns for all these genes at different time points during viral infection. The tolerant genotype exhibited higher expression levels of all , , , , 1, and 2 genes compared to the susceptible genotype, with the 1 gene showing the highest transcript levels. These findings provide the initial evidence of the potential roles of s, , , , 1, 2, and 3 in responding to the stress induced by BCMV in common bean. The results presented herein will serve as a valuable resource for guiding future breeding studies aimed at addressing BCMV-induced stress in common bean cultivation.

中文翻译：

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探讨BCMV感染过程中菜豆的差异表达基因

菜豆普通花叶病毒 (BCMV) 是影响菜豆的重要病原体，导致大量产量损失和作物品质下降。为了减轻 BCMV 攻击，某些基因，包括二酰甘油激酶基因 ()、与防御和应激反应相关的基因 (、 和 )，以及与植物防御相关的基因 (1、2 和 3)，在普通豆的各种应激反应。本研究调查了 BCMV 感染条件下不同菜豆基因型叶片中 1235a5b6、、、、、1、2、3 基因的表达水平。通过实时定量 PCR 分析，我们观察到所有这些基因在病毒感染过程中不同时间点的不同表达模式。与易感基因型相比，耐受基因型的所有 、 、 、 、 1 和 2 基因均表现出较高的表达水平，其中 1 基因显示出最高的转录水平。这些发现提供了 s、、、、、、1、2 和 3 在响应普通豆中 BCMV 诱导的胁迫方面的潜在作用的初步证据。本文提出的结果将作为指导未来育种研究的宝贵资源，旨在解决菜豆种植中 BCMV 诱导的胁迫问题。