The present study was undertaken to examine the role of IL-4 (− 590 C/T) (rs2243250) and IL-6 (− 174G/C) (rs1800795) polymorphism and the serum levels of IL-4 and IL-6 in chronic kidney disease (CKD). The IL-4 (− 590C/T) and IL-6 (− 174 G/C) polymorphisms were genotyped in 132 CKD patients and 161 controls using PCR–RFLP. Serum IL-4 and IL-6 quantifications were performed by ELISA. Significant susceptible associations of CT genotype (OR = 4.56; p < 1.84 × 10–9) and T allele (OR = 1.56; p < 0.010) of IL-4 (− 590C/T) and CC genotype (OR = 2.63; p < 0.032) of IL-6 (− 174G/C) were observed for CKD. The CC genotype (OR = 0.27; p < 9.314 × 10–7) and C allele (OR = 0.63; p < 0.010) of IL-4 (− 590 C/T) revealed strong protective associations. Five-fold increased levels were observed for both IL-6 (p < 0.0001) and IL-4 (p < 0.0043) cytokines in CKD patients than the controls. The IL-4 serum levels (pg/ml) increased significantly in patients with CT and TT genotypes of IL-4 (− 590 C/T) than the controls (6.18 ± 1.80 vs. 3.33 ± 0.48 and 6.14 ± 1.96 vs. 3.21 ± 0.56 respectively). For IL-6 (− 174 G/C) polymorphism, the patients with CC genotype (6.50 ± 1.30 vs. 3.49 ± 1.39) revealed with higher IL-6 serum levels followed by GC genotype (5.00 ± 1.91 vs. 4.01 ± 1.74). The genotypes of IL-4 (590 C/T) and IL-6 (174 G/C) polymorphisms contribute differential susceptibility in south Indian CKD patients. A fivefold increased serum levels of IL-4 (anti-inflammatory) and IL-6 (pro- and anti-inflammatory) cytokines were documented in CKD patients. There observed an opposite trend in disease association for these two cytokines and associated SNPs with CKD in south India.

中文翻译：

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南印度 CKD 患者中 IL-4 (− 590 C/T) 和 IL-6 (− 174 G/C) 基因多态性的关联

本研究旨在检查 IL-4 (− 590 C/T) (rs2243250) 和 IL-6 (− 174G/C) (rs1800795) 多态性的作用以及 IL-4 和 IL-6 的血清水平。慢性肾脏病（CKD）。使用 PCR-RFLP 对 132 名 CKD 患者和 161 名对照者的 IL-4 (− 590C/T) 和 IL-6 (− 174 G/C) 多态性进行了基因分型。通过 ELISA 进行血清 IL-4 和 IL-6 定量。 IL-4 (− 590C/T) 的 CT 基因型 (OR = 4.56; p < 1.84 × 10–9) 和 T 等位基因 (OR = 1.56; p < 0.010) 和 CC 基因型 (OR = 2.63; p < 0.010) 之间存在显着的易感相关性观察到 CKD 的 IL-6 (− 174G/C) < 0.032)。 IL-4 (− 590 C/T) 的 CC 基因型 (OR = 0.27; p < 9.314 × 10–7) 和 C 等位基因 (OR = 0.63; p < 0.010) 显示出很强的保护性关联。与对照组相比，CKD 患者的 IL-6 (p < 0.0001) 和 IL-4 (p < 0.0043) 细胞因子水平增加了五倍。 IL-4 CT 和 TT 基因型 (− 590 C/T) 患者的 IL-4 血清水平 (pg/ml) 显着高于对照组（6.18 ± 1.80 vs. 3.33 ± 0.48 和 6.14 ± 1.96 vs. 3.21）分别为±0.56）。对于 IL-6 (− 174 G/C) 多态性，CC 基因型患者（6.50 ± 1.30 vs. 3.49 ± 1.39）显示出较高的 IL-6 血清水平，其次是 GC 基因型患者（5.00 ± 1.91 vs. 4.01 ± 1.74） 。 IL-4 (590 C/T) 和 IL-6 (174 G/C) 多态性的基因型导致南印度 CKD 患者的不同易感性。 CKD 患者的血清 IL-4（抗炎）和 IL-6（促炎和抗炎）细胞因子水平增加了五倍。在印度南部，观察到这两种细胞因子和相关 SNP 与 CKD 的疾病关联呈相反趋势。