Codium fragile has been traditionally used in oriental medicine to treat enterobiasis, dropsy, and dysuria, and it has been shown to possess many biological properties. Atopic dermatitis (AD) is one of the types of skin inflammation and barrier disruption, which leads to chronic inflammatory skin diseases. In the current investigation, the protective effects of C. fragile extract (CFE) on anti-inflammation and skin barrier improvement were investigated. In LPS-stimulated RAW 264.7 cells, nitric oxide generation and the expression levels of interleukin (IL)-1β, IL-4, IL-6, iNOS, COX-2, and tumor necrosis factor-alpha (TNF)-α were reduced by CFE. CFE also inhibited the phosphorylation of NF-κB-p65, ERK, p-38, and JNK. Additionally, CFE showed inhibitory activity on TSLP and IL-4 expression in HaCaT cells stimulated with TNF-α/interferon- gamma (IFN-γ). Enhanced expression of factors related to skin barrier function, FLG, IVL, and LOR, was confirmed. These findings implied that CFE may be used as a therapeutic agent against AD due to its skin barrier-strengthening and anti-inflammatory activities, which are derived from natural marine products.

中文翻译：

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脆钠提取物对特应性皮炎巨噬细胞和人角质形成细胞的体外抗炎和皮肤保护作用。

脆钠传统上在东方医学中用于治疗蛲虫病、水肿和排尿困难，并且已被证明具有许多生物学特性。特应性皮炎（AD）是皮肤炎症和屏障破坏的一种类型，可导致慢性炎症性皮肤病。在当前的研究中，研究了脆念珠菌提取物（CFE）的抗炎和改善皮肤屏障的保护作用。在 LPS 刺激的 RAW 264.7 细胞中，一氧化氮的生成以及白细胞介素(IL)-1β、IL-4、IL-6、iNOS、COX-2和肿瘤坏死因子-α (TNF)-α的表达水平降低由 CFE 提供。 CFE 还抑制 NF-κB-p65、ERK、p-38 和 JNK 的磷酸化。此外，CFE 对 TNF-α/干扰素-γ (IFN-γ) 刺激的 HaCaT 细胞中的 TSLP 和 IL-4 表达显示出抑制活性。证实了与皮肤屏障功能相关的因子 FLG、IVL 和 LOR 的表达增强。这些发现表明，CFE 可以作为 AD 的治疗剂，因为它具有增强皮肤屏障和抗炎活性，这些活性来自天然海产品。