Electrospun nanofibers endowed with efficient antibacterial properties have found widespread biomedical applications. Poly (Ɛ-caprolactone) (PCL) nanofibers are one of the most popular due to their distinct mechanical and chemical properties, especially in biotechnological applications. Herein, PCL hybrid electrospun nanofibers were fabricated successfully via the electrospinning method, and preparation parameters were optimized at various concentrations of cetyltrimethylammonium bromide (CTAB)-modified montmorillonite (CTAB-Mt) and silver nanoparticles (AgNP) as a nanofillers for the first time. The nanofibers were characterized using SEM, FTIR, XPS, diameter distribution and contact angle measurements. It was found that 0.5% (w/w) CTAB-Mt and 1.0% AgNP (w/w) in the 15% (w/w) in PCL nanofiber is an ideal condition to obtain bead-free and uniform polymer nanofibers. Finally, based on the disc diffusion method, the antibacterial properties of nanofibers were tested against four Gram-negative and Gram-positive bacteria. The cytotoxic nature of the PCL hybrid nanofiber was studied against SV-40 cells using resazurin assay.

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中文翻译：

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聚(Ɛ-己内酯)-CTAB蒙脱石-银纳米粒子：具有体外抗菌活性的混合电纺纳米纤维

静电纺丝纳米纤维具有高效的抗菌性能，在生物医学领域有着广泛的应用。聚（Ɛ-己内酯）（PCL）纳米纤维因其独特的机械和化学性能而成为最受欢迎的纳米纤维之一，特别是在生物技术应用中。在此，通过静电纺丝方法成功制备了PCL杂化电纺纳米纤维，并首次在不同浓度的十六烷基三甲基溴化铵（CTAB）改性蒙脱土（CTAB-Mt）和银纳米颗粒（AgNP）作为纳米填料时优化了制备参数。使用 SEM、FTIR、XPS、直径分布和接触角测量对纳米纤维进行表征。结果发现，15%（w/w）PCL纳米纤维中0.5%（w/w）CTAB-Mt和1.0% AgNP（w/w）是获得无珠且均匀的聚合物纳米纤维的理想条件。最后，基于纸片扩散法，测试了纳米纤维对四种革兰氏阴性菌和革兰氏阳性菌的抗菌性能。使用刃天青测定研究了 PCL 混合纳米纤维对 SV-40 细胞的细胞毒性。

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