Phospholipids are important components of biological membranes, participating in various biological processes, including plant development and responses to biotic and abiotic stresses. A previous study showed that mutation of the rice OsCDS5 (CDP-DAG Synthase) gene alters lipid metabolism, causing enhanced abiotic stress responses, yellowing of leaves at the seedling stage and delayed plant development. Here, we observed that the Oscds5 mutant shows enhanced resistance to rice blast, bacterial blight and bacterial leaf streak. Mutation of OsCDS5 promotes production of reactive oxygen species and increases the expression level of multiple defence-related genes. Transcriptomic analyses indicate that genes involved in responses to stress, biotic/abiotic stimuli and metabolic processes are highly upregulated and enriched in mutant Oscds5. Metabolomic analyses showed that differential metabolites were enriched in the lipid metabolic and tryptophan metabolic pathways. The decreased level of phosphatidylinositol and increased level of serotonin probably contribute to enhanced disease resistance of the Oscds5 mutant. Taken together, mutation of OsCDS5 enhances abiotic and biotic stress responses, and OsCDS5 may be a promising target for genetic engineering to enhance the resilience of rice to abiotic and biotic stresses simultaneously.

中文翻译：

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OsCDS5 突变赋予水稻广谱抗病性

磷脂是生物膜的重要组成部分，参与各种生物过程，包括植物发育以及对生物和非生物胁迫的反应。先前的一项研究表明，水稻OsCDS5（C DP- D AG S合酶）基因的突变会改变脂质代谢，导致非生物胁迫反应增强、苗期叶片黄化并延迟植物发育。在这里，我们观察到Oscds5突变体对稻瘟病、白叶枯病和细菌性叶斑病表现出增强的抗性。OsCDS5的突变促进活性氧的产生并增加多种防御相关基因的表达水平。转录组分析表明，参与应激反应、生物/非生物刺激和代谢过程的基因在突变体Oscds5中高度上调和富集。代谢组学分析表明差异代谢物在脂质代谢和色氨酸代谢途径中富集。磷脂酰肌醇水平的降低和血清素水平的升高可能有助于增强Oscds5突变体的抗病性。综上所述，OsCDS5的突变增强了非生物和生物胁迫反应，OsCDS5可能是基因工程的一个有希望的目标，以同时增强水稻对非生物和生物胁迫的恢复能力。