The negative effects of plant protection chemicals on the environment and human health have led scientists to research alternative control methods. Fungi, especially Trichoderma have an important place in biological control, one of the most common alternative methods. Licensed as an agricultural product, it has been effectively used in agricultural lands. This study aimed to evaluate long-lasting carriers of the ET 4 and ET 14 Trichoderma harzianum isolates, proven effective against different pathogens in previous studies, to allow licensing for their commercial and mass production in the industry. The efficacy of ET 4 and ET 14 isolates against Alternaria solani (isolate ET 66) was initially confirmed by testing in vitro and in vivo conditions. Then, spores of the fungus isolate developed on potato dextrose agar (PDA) were transferred to five different liquid media (soybean oil, neem oil, canola oil, paraffin oil, and glycerine) prepared as the carrier formulations. They were kept at room (22 ℃) and refrigerator (+4 ℃) temperatures for 10 months. The viability tests of the bioagent fungus were performed by sowing into PDA from the monthly samples taken from the formulations. In addition, at the end of the tenth month, the efficacy of the bioagents was tested against the pathogenic fungus in vitro and in vivo conditions. Even though the most successful carrier was neem oil with a very intense bioagent development, the bioagent maintained its viability in all carriers even at the end of the 10th month, with percent inhibition rates varying between 37.85% and 52.33% in vitro and between 14.26% and 3.95% in vivo conditions. It was concluded that paraffin, glycerin, and especially neem oil were good carriers for the T. harzianum bioagent, that the shelf life could be extended even more with further studies, and that it could be licensed as a biopesticide after toxicological and ecotoxicological evaluations.

植保化学品对环境和人类健康的负面影响促使科学家研究替代控制方法。真菌，尤其是木霉在生物防治中占有重要地位，是最常见的替代方法之一。作为农产品获得许可，已在农田中有效使用。本研究旨在评估 ET 4 和 ET 14哈茨木霉分离株的持久载体，在之前的研究中证明对不同病原体有效，以便为其在行业中进行商业化和大规模生产获得许可。 ET 4 和 ET 14 分离株对抗黑斑病（分离株 ET 66）的功效最初通过体外和体内条件测试得到证实。然后，将在马铃薯葡萄糖琼脂（PDA）上发育的真菌分离物的孢子转移到作为载体制剂制备的五种不同的液体介质（大豆油、印楝油、菜籽油、石蜡油和甘油）中。它们在室温（22℃）和冰箱（+4℃）温度下保存了10个月。通过将每月从制剂中采集的样品播种到 PDA 中来进行生物剂真菌的活力测试。此外，在第十个月末，在体外和体内条件下测试了生物制剂针对病原真菌的功效。尽管最成功的载体是印楝油，具有非常强烈的生物制剂开发，但该生物制剂即使在第 10 个月末也能在所有载体中保持其活力，体外抑制率在 37.85% 至 52.33% 之间变化，在体外抑制率在 14.26% 之间变化。和 3.95%体内条件。结论是石蜡、甘油，特别是印楝油是哈茨木霉生物剂的良好载体，通过进一步研究可以进一步延长其保质期，并且经过毒理学和生态毒理学评估后可以作为生物农药许可。