The aim of this study was to determine the changes occurring in the wood cellulose of the fast-growing poplar (Populus deltoides × maximowiczii) under the influence of steam explosion (SE) pretreatment. Cellulose from native wood and after pretreatment at 160 and 205 °C was isolated. Cellulose polymerization degree by size exclusion chromatography (SEC) and cellulose crystallinity index by Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) were determined. The profiles of sugars in the native wood and in the solid fraction after pretreatment (using the acid hydrolysis method) were also determined. In addition, the profile of monosaccharides in the liquid fraction obtained after steam explosion and in the liquid fraction after acid hydrolysis of the oligosaccharides were investigated using high-performance liquid chromatography (HPLC). This allowed to determine the change in the yield of hexoses and pentoses in the studied material.

The behavior of cellulose in wood subjected to steam explosion at 160 and 205 °C and isolated by the Kürschner–Hoffer method was studied by determining the absorption bands of FTIR-ATR spectra. The lateral order index (LOI) of cellulose was calculated from the ratio of the intensity of the corresponding absorption bands A₁₄₂₂/A₈₉₆ cm⁻¹. Total crystallinity index (TCI) of cellulose was calculated from the ratio of the intensity of absorption bands A₁₃₇₂/A₂₉₀₀ cm⁻¹. TCI of Kürschner-Hoffer cellulose isolated from wood subjected to steam explosion at 160 and 205 °C decreased by 5.6 and 5.0%, respectively, with regard to the applied temperature. LOI increased in cellulose isolated from wood subjected to steam explosion at 160 °C (by 0.7%) and at 205 °C (by 19.2%) in relation to the index of cellulose isolated from native wood. Kürschner–Hoffer cellulose isolated from wood subjected to steam explosion at 160 and 205 °C exhibited, respectively, a reduced degree of polymerization of about 11% and about 8%. Polydispersity index in Kürschner–Hoffer cellulose was 1% lower after both pretreatments than native sample.

本研究的目的是确定速生杨树（Populus deltoides  ×  maximowiczii）的木材纤维素在蒸汽爆炸（SE）预处理的影响下发生的变化。从天然木材中分离出纤维素，并在 160 和 205 °C 下进行预处理。通过尺寸排阻色谱法（SEC）测定纤维素聚合度，通过傅里叶变换红外光谱-衰减全反射率（FTIR-ATR）测定纤维素结晶度指数。还测定了天然木材和预处理后（使用酸水解方法）的固体部分中的糖分布。此外，使用高效液相色谱（HPLC）研究了蒸汽爆炸后获得的液体级分和寡糖酸水解后的液体级分中的单糖谱。这使得可以确定所研究材料中己糖和戊糖产量的变化。

通过确定 FTIR-ATR 光谱的吸收带，研究了在 160 和 205 °C 下经受蒸汽爆炸并通过 Kürschner-Hoffer 方法分离的木材中纤维素的行为。纤维素的侧序指数(LOI)由相应吸收带的强度比A ₁₄₂₂ /A ₈₉₆  cm ^-1计算。由吸收带强度之比A ₁₃₇₂ /A ₂₉₀₀  cm ^-1算出纤维素的总结晶度指数(TCI) 。从木材中分离出的 Kürschner-Hoffer 纤维素在 160 和 205 °C 下经受蒸汽爆炸后，其 TCI 相对于所施加的温度分别降低了 5.6 和 5.0%。与从天然木材中分离的纤维素的指数相比，在 160°C（增加 0.7%）和 205°C（增加 19.2%）的蒸汽爆炸下从木材中分离的纤维素的 LOI 增加。从木材中分离出的 Kürschner-Hoffer 纤维素在 160 和 205 °C 下经受蒸汽爆炸，聚合度分别降低了约 11% 和约 8%。经过两种预处理后，Kürschner-Hoffer 纤维素的多分散指数比天然样品低 1%。