Anesthetics have varying physiological effects, but most notably alter ion channel kinetics. Alfaxalone is a rapid induction and washout neuroactive anesthetic, which potentiates γ-aminobutyric acid (GABA)-activated GABA_A receptor (GABA_A-R) currents. This study aims to identify any long-term effects of alfaxalone sedation on pyramidal neuron action potential and GABA_A-R properties, to determine if its impact on neuronal function can be reversed in a sufficiently short timeframe to allow for same-day electrophysiological studies in goldfish brain. The goldfish (Carassius auratus) is an anoxia-tolerant vertebrate and is a useful model to study anoxia tolerance mechanisms. The results show that alfaxalone sedation did not significantly impact action potential properties. Additionally, the acute application of alfaxalone onto naive brain slices caused the potentiation of whole-cell GABA_A-R current decay time and area under the curve. Following whole-animal sedation with alfaxalone, a 3-h wash of brain slices in alfaxalone-free saline, with saline exchanged every 30 min, was required to remove any potentiating impact of alfaxalone on GABA_A-R whole-cell currents. These results demonstrate that alfaxalone is an effective anesthetic for same-day electrophysiological experiments with goldfish brain slices.

中文翻译：

---

阿法沙酮对金鱼锥体神经元动作电位特性或 GABAA 受体电流没有长期影响

麻醉剂具有不同的生理效应，但最显着的是改变离子通道动力学。 Alfaxalone 是一种快速诱导和冲洗的神经活性麻醉剂，可增强 γ-氨基丁酸 (GABA) 激活的 GABA _A受体 (GABA _A -R) 电流。本研究旨在确定阿法沙酮镇静对锥体神经元动作电位和 GABA _A -R 特性的长期影响，以确定其对神经元功能的影响是否可以在足够短的时间内逆转，以便在当天进行电生理学研究金鱼的大脑。金鱼（Carassius auratus）是一种耐缺氧脊椎动物，是研究缺氧耐受机制的有用模型。结果表明，阿法沙酮镇静并没有显着影响动作电位特性。此外，将阿法沙酮急性应用到初始脑切片上会导致全细胞 GABA _A -R 电流衰减时间和曲线下面积增强。用阿法沙酮对整个动物进行镇静后，需要用不含阿法沙酮的盐水清洗脑切片 3 小时，每 30 分钟更换一次盐水，以消除阿法沙酮对 GABA _A -R 全细胞电流的任何潜在影响。这些结果表明，阿法沙酮是金鱼脑片当天电生理实验的有效麻醉剂。