Abstract

N⁶-methyladenosine (m⁶A) is one of the most common modifications in both eukaryotic and prokaryotic mRNAs. It has been experimentally confirmed that m⁶A methylation is involved in the regulation of stability and translation of various mRNAs. Until recently, the majority of m⁶A-related studies have been focused on the cytoplasmic functions of this modification. Here, we review new data on the role of m⁶A in several key biological processes taking place in the cell nucleus, such as transcription, chromatin organization, splicing, nuclear-cytoplasmic transport, and R-loop metabolism. Based on analysis of these data, we suggest that m⁶A methylation of nuclear RNAs is another level of gene expression regulation which, together with DNA methylation and histone modifications, controls chromatin structure and functioning in various biological contexts.

中文翻译：

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N6-甲基腺苷在核 RNA 中的功能

摘要

N ⁶ -甲基腺苷 (m ⁶ A) 是真核和原核 mRNA 中最常见的修饰之一。实验证实m ⁶ A甲基化参与多种mRNA稳定性和翻译的调节。直到最近，大多数 m ⁶ A 相关研究都集中在这种修饰的细胞质功能上。在这里，我们回顾了 m ⁶ A 在细胞核中发生的几个关键生物过程中的作用的新数据，例如转录、染色质组织、剪接、核质运输和 R 环代谢。基于对这些数据的分析，我们认为核 RNA 的 m ⁶ A 甲基化是基因表达调控的另一个水平，它与 DNA 甲基化和组蛋白修饰一起控制染色质结构和在各种生物环境中的功能。